Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.
TAP tandem affinity purification of bait and identification of prey by mass spectrometry.
Source was nuclear extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.
Source was embryonic nuclear extract of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.
Source was embryonic nuclear extract of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.
Two-hybrid system: yeast GAL4-BD/GAL4-AD
Positive control.
Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).
Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.
Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.
Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.
Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.
Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.
Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.
coordinates relative to Cp190-PA
V7A; coordinates relative to Cp190-PA; mutation within the context of BTB interaction domain residues 1-126
deletion of aa 220-232; disruption occurs only within the context of Cp190 BTB domain 1-126 as bait, not full length Cp190.
deletion of aa 114-164 is able to interact with all other Cp190 BTB interaction residue mutations(15,19,114,117) with the exception of V7A and L118A.
Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was from cDNA library of 154 Drosophila C2H2 domain proteins).
Two-hybrid system: yeast GAL4-BD/GAL4-AD
Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from wild-type, nuclear embryonic extract.
within the context of aa 1-293; coordinates relative to Cp190-PA
Two-hybrid system: yeast GAL4-BD/VP16-AD
Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).
