Source was cell extract of S2 cell line; bait produced from endogenous gene; prey produced from endogenous gene.

This interaction is RNA-dependent (disrupted by treatment with micrococcal nuclease).

Proteins were labeled with [35]S and immunoprecipitated using the 8G6 monoclonal antibody.

Bound proteins separated by two dimensional electrophoresis and detected by silver staining.

Source was cell extract of S2 cell line; bait produced from endogenous gene; prey produced from endogenous gene.

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey from ovary cDNA expression library).

Two-hybrid system: yeast LexA-BD/GAL4-AD

Four of 183 positive clones interacting with sqd bait corresponded to Hrb27C clones.

Source was adult female ovaries of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

Hrb27C specifically coimmunoprecipitates with sqd (but not spn-F) in an RNA-dependent way (interaction disrupted by RNaseA).

Source was adult female ovaries of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

Copurified proteins were identified only in sqd immunoprecipitation, and not in the negative control (dl immunoprecipitation).