FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
slmb, ex
FlyBase ID
FBig0000102574
Interaction Network
Interactions Browser links
slmb network
ex network
Reported Interactions
FBrf0225065-2.coIP.WB
Reference
Ribeiro et al., 2014
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
ex 

Ex

bait

FLAG tag

ex 
slmb 

Slmb

prey

V5 tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
ex 
N-terminal region
sufficient binding region
aa 1-468
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

ex and slmb (ΔF-box) were cotransfected with the intracellular domain of crb (which destabilizes ex protein). Cells were treated with MG132 (proteasome inhibitor) for 4 hr before lysis.

(Ribeiro et al., 2014)

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Ribeiro et al., 2014)
FBrf0225065-3.coIP.MS
Reference
Ribeiro et al., 2014
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
ex 

Ex

bait

FLAG tag

ex 
slmb 

Slmb

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
ex 
N-terminal region
sufficient binding region
aa 1-468
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Ribeiro et al., 2014)
FBrf0225065-5.coIP.WB
Reference
Ribeiro et al., 2014
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
slmb 

Slmb

bait

FLAG tag

slmb 
ex 

ex

prey

V5 tag

ex 
ex 
ex 
ex 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
ex 
N-terminal region
sufficient binding region
aa 1-468
ex 
Slmb recognition site
necessary binding region
aa 451-468

specifically, TSGIVS

ex 
Slmb recognition site residue 453
mutation disrupting interaction
aa 453

S453A

ex 
Slmb recognition site residue 462
mutation decreasing interaction
aa 462

S462A

slmb 
WD40 repeat motif
mutation disrupting interaction
aa 417

R417A, coordinates relative to slmb-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

slmb fails to interact with Mer or Moe.

(Ribeiro et al., 2014)

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Ribeiro et al., 2014)
FBrf0227055-1.coIP.WB
Reference
Zhang et al., 2015
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
ex 

Ex

bait

FLAG tag

ex 
ex 
slmb 

Slmb

prey

HA tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
ex 
C-terminal region C2
sufficient binding region
aa 912-1164
ex 
wts phosphorylation site
mutation increasing interaction
aa 1116

S1116A

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Zhang et al., 2015)

Cotransfection of slmb promotes ex degradation, while slmb RNAi depletion inhibits polyubiquitination of ex.

(Zhang et al., 2015)

Co-transfection of wts antagonizes the coimmunoprecipitation of slmb with ex; mutation of the wts target phosphorylation site in ex increases the ex-slmb interaction.

(Zhang et al., 2015)
FBrf0227055-2.PD.WB
Reference
Zhang et al., 2015
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
slmb 

Slmb

bait

GST tag

ex 

Ex

prey

His tag

ex 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
ex 
C-terminal region C2
sufficient binding region
aa 912-1164
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

(Zhang et al., 2015)
FBrf0241288-10.coIP.WB
Reference
Wang et al., 2019
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2R+
Participants
Corresponds to
Reported as
Role
Note
ex 

Ex

bait

FLAG tag

ex 
ex 
ex 
slmb 

Slimb

prey

HA tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
ex 
C2 domain
sufficient binding region
aa 912-1164
ex 
C3 domain
sufficient binding region
aa 1168-1427
ex 
N-terminal domain
sufficient binding region
aa 1-653
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Wang et al., 2019)
FBrf0241288-11.PD.WB
Reference
Wang et al., 2019
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2R+
Participants
Corresponds to
Reported as
Role
Note
slmb 

Slimb

bait

GST tag

ex 

Ex

prey

His tag

ex 
ex 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
ex 
C2 domain
sufficient binding region
aa 912-1164
ex 
C3 domain
sufficient binding region
aa 1168-1427
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from S2 cell extract.

(Wang et al., 2019)
FBrf0243590-4.coIP.WB
Reference
Fulford et al., 2019
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-ATCC
Participants
Corresponds to
Reported as
Role
Note
slmb 

Slmb

bait

FLAG tag

ex 

Ex

prey

V5 tag

ex 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
ex 
interaction domain
sufficient binding region
aa 1-468
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Fulford et al., 2019)

The slmb-ex interaction was detected under conditions where either the intracellular domain of crb, dco, CkIα or gish-PI were coexpressed.

(Fulford et al., 2019)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (4)