FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
foxo, 14-3-3ε
FlyBase ID
FBig0000102680
Interaction Network
Interactions Browser links
foxo network
14-3-3ε network
Reported Interactions
FBrf0207166-1.coIP.MS
Reference
Nielsen et al., 2008
Description
physical association
Assay
Collection
Source/Stage
adult head / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
foxo 

Foxo

bait

FLAG tag

14-3-3ε 

14-3-3ε

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adult heads of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Nielsen et al., 2008)
FBrf0207166-3.coIP.WB
Reference
Nielsen et al., 2008
Description
physical association
Assay
Collection
Source/Stage
adult head / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
foxo 

Foxo

bait

FLAG tag

14-3-3ε 

14-3-3ε

prey

Myc tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adult heads of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene or tagged transgenic construct.

(Nielsen et al., 2008)

The foxo-14-3-3ε is disrupted by paraquat treatment.

(Nielsen et al., 2008)
FBrf0216604-6.coIP.MS
Reference
Glatter et al., 2011
Description
physical association
Assay
Collection
PPI_InR-Tor
Source/Stage
Cell line used
Kc167
Participants
Corresponds to
Reported as
Role
Note
foxo 

foxo

bait

GFP tag

14-3-3ε 

14-3-3epsilon

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

SAINT score = 1

(Glatter et al., 2011)

Source was cell extract of Kc167 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Glatter et al., 2011)
FBrf0233454-150.coIP.MS
Reference
Vinayagam et al., 2016
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2R+
Participants
Corresponds to
Reported as
Role
Note
foxo 

foxo

bait

TAP tag

14-3-3ε 

14-3-3epsilon

prey

SAINT score = 1

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2R+ cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Vinayagam et al., 2016)

Observed in two of three conditions tested: 10 minutes and 30 minutes after insulin stimulation.

(Vinayagam et al., 2016)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (3)