Two-hybrid system: yeast GAL4-BD/GAL4-AD

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

Exact mutations to the necessary SEDPLCYSPIHVMDDEGL sequence were not specified.

Authors knocked down endogenous BEAF-32 by RNAi and expressed wild-type or mutant versions of BEAF-32 with synonymous mutations in the first 800bp at all available degenerate codons to allow the transfected construct to evade RNAi.

Source was nuclear extract of S2 cells; bait produced from endogenous gene; prey produced from transfected construct.

Exact mutations to the necessary SEDPLCYSPIHVMDDEGL sequence were not specified.

BEAF-32, Cp190 and Chro were incubated, alone or in combination, with genomic DNA spanning an insulator at the Tudor-SN locus.

Cp190 has no observed DNA binding activity on its own, but it supershifts the BEAF-32 protein-DNA complex.

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion pr