FB2026_02 , released June 18, 2026
FB2026_02 , released June 18, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Met, ftz-f1
FlyBase ID
FBig0000104867
Interaction Network
Interactions Browser links
Met network
ftz-f1 network
Reported Interactions
FBrf0215576-1.PD.WB
Reference
Dubrovsky et al., 2011
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
Met 

MET

bait

GST tag

ftz-f1 

FTZ-F1

prey

V5 tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction between ftz-f1 and Met was enhanced following treatment of S2 cells with methoprene.

(Dubrovsky et al., 2011)

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Dubrovsky et al., 2011)
FBrf0217595-1.2H
Reference
Bernardo and Dubrovsky, 2012
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
Met 

Met

bait

fused to GAL4 DNA-binding domain

Met 
Met 
ftz-f1 

ftz-f1

prey

fused to p65 activation domain

ftz-f1 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
ftz-f1 
C-terminal domain
mutation decreasing interaction
aa 789-803

coordinates relative to ftz-f1-PA

Met 
interaction domain residue 554
mutation decreasing interaction
aa 554

L554A

Met 
interaction domain residues 554,632,635,637,639,647
mutation disrupting interaction
aa 554,632,635,637,639,647

mutation L554A and unspecified mutations in glutamine-rich region

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was S2 cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Bernardo and Dubrovsky, 2012)

Mutations in glutamine-rich region alone have little effect on interaction.

(Bernardo and Dubrovsky, 2012)
FBrf0257441-1.NMR
Reference
Kolonko-Adamska et al., 2023
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Met 

Met

neutral component

His tag

Met 
Met 

Met

neutral component

His tag

Met 
Met 

Met

neutral component

His tag

ftz-f1 
ftz-f1 

Ftz-F1

neutral component

His tag

ftz-f1 

Ftz-F1

neutral component

His tag

ftz-f1 
ftz-f1 

Ftz-F1

neutral component

His tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Met 
C terminal domain
sufficient binding region
aa 509-716

coordinates relative to Met-PA

ftz-f1 
Ligand binding domain
sufficient binding region
aa 786-1027
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; proteins produced as recombinant fusion proteins in bacterial system.

(Kolonko-Adamska et al., 2023)
FBrf0257441-2.NMR
Reference
Kolonko-Adamska et al., 2023
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Met 

Met

neutral component
Met 
Met 

Met

neutral component
Met 
Met 

Met

neutral component
ftz-f1 
ftz-f1 

Ftz-F1

neutral component

His tag

ftz-f1 

Ftz-F1

neutral component

His tag

ftz-f1 
ftz-f1 

Ftz-F1

neutral component

His tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Met 
NR box
sufficient binding region
aa LERIVLYLIENLQKSVDSA

coordinates relative to Met-PA

ftz-f1 
Ligand binding domain
sufficient binding region
aa 786-1027
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; Met peptide chemically synthesized; ftz-f1 produced as a recombinant fusion protein in bacterial system.

(Kolonko-Adamska et al., 2023)
FBrf0257441-4.ITC
Reference
Kolonko-Adamska et al., 2023
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
ftz-f1 

Ftz-F1

bait

His tag

ftz-f1 
Met 

Met

prey
Met 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
ftz-f1 
Ligand binding domain
sufficient binding region
aa 786-1027
Met 
NR box
sufficient binding region
aa LERIVLYLIENLQKSVDSA

coordinates relative to Met-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey chemically synthesized.

(Kolonko-Adamska et al., 2023)

Kd 9.9 +/- 0.5 uM

(Kolonko-Adamska et al., 2023)
FBrf0257441-5.PD.WB
Reference
Kolonko-Adamska et al., 2023
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Met 

Met

bait

FLAG-YFP tag

Met 
ftz-f1 

Ftz-F1

prey

CFP tag

ftz-f1 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
ftz-f1 
Ligand binding domain
sufficient binding region
aa 786-1027
Met 
C terminal domain
sufficient binding region
aa 509-716

coordinates relative to Met-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of COS-7 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Kolonko-Adamska et al., 2023)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (3)