FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Rel, akirin
FlyBase ID
FBig0000105001
Interaction Network
Interactions Browser links
Rel network
akirin network
Reported Interactions
FBrf0226504-2.coIP.WB
Reference
Bonnay et al., 2014
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
akirin 

Akirin

bait

V5 tag

Rel 

Relish

prey

FLAG tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

The akirin-Rel is stronger after bacterial stimulation of cells with heat-killed E. coli.

(Bonnay et al., 2014)

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Bonnay et al., 2014)
FBrf0226504-3.coIP.WB
Reference
Bonnay et al., 2014
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
Rel 

Relish

bait

FLAG tag

akirin 

Akirin

prey

V5 tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Bonnay et al., 2014)
FBrf0226504-6.coIP.WB
Reference
Bonnay et al., 2014
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
Rel 

Relish

bait

FLAG tag

akirin 

Akirin

prey

His tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait was purified from transfected S2 cell extract; prey produced as a recombinant fusion protein in bacterial system.

(Bonnay et al., 2014)
FBrf0245575-2.coIP.WB
Reference
Cammarata-Mouchtouris et al., 2020
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
akirin 

Ak

bait

V5 tag

Rel 

Rel

prey

HA tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Knock-down of hyd decreases the akirin-Rel interaction.

(Cammarata-Mouchtouris et al., 2020)

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Cammarata-Mouchtouris et al., 2020)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (2)