FB2026_02 , released June 18, 2026
Physical Interaction report
Open Close
General Information
Interaction Type
physical association
Interacting Genes
robo2, robo1
FlyBase ID
FBig0000106638
Interaction Network
Interactions Browser links
robo2 network
robo1 network
Reported Interactions
FBrf0132431-4.PD.A
Reference
Simpson et al., 2000
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
robo1 

Robo

bait

GST tag

robo1 
robo2 

Robo2

prey

[35]S label

robo2 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
robo1 
cytoplasmic domain
sufficient binding region
robo2 
cytoplasmic domain
sufficient binding region
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

(Simpson et al., 2000)
FBrf0132431-5.PD.A
Reference
Simpson et al., 2000
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
robo2 

Robo2

bait

GST tag

robo2 
robo1 

Robo

prey

[35]S label

robo1 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
robo1 
cytoplasmic domain
sufficient binding region
robo2 
cytoplasmic domain
sufficient binding region
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

(Simpson et al., 2000)
FBrf0229066-2.coIP.WB
Reference
Evans et al., 2015
Description
physical association
Assay
Collection
Source/Stage
larval nervous system / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
robo1 

Robo1

bait

Myc tag

robo2 

Robo2

prey

HA tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Transgenic constructs were expressed pan-neuronally using elav driver.

(Evans et al., 2015)

Source was embryos of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Evans et al., 2015)
FBrf0229066-4.coIP.WB
Reference
Evans et al., 2015
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2R+
Participants
Corresponds to
Reported as
Role
Note
robo1 

Robo1

bait

Myc tag

robo2 

Robo2

prey

HA tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2R+ cell line; bait produced from transfected construct; prey produced from transfected construct.

(Evans et al., 2015)
FBrf0229066-6.coIP.WB
Reference
Evans et al., 2015
Description
physical association
Assay
Collection
Source/Stage
larval nervous system / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
robo2 

Robo2

bait

Myc tag

robo1 

Robo1

prey

HA tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Transgenic constructs were expressed pan-neuronally using elav driver.

(Evans et al., 2015)

Source was embryos of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Evans et al., 2015)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (2)