Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).
Two-hybrid system: yeast GAL4-BD/GAL4-AD
Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from wild-type embryonic extract.
Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced by in vitro translation.
Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.
Source was fixed S2 cells; bait produced from transfected construct; prey produced from transfected construct.
Bait protein was fused to an VSV-G protein, which targets the chimera to the plasma membrane. The ability of this chimera to recruit prey protein to plasma membrane was taken as evidence of direct physical interaction.
Interaction in vitro; protein produced as a recombinant fusion proteins in bacterial system or chemically synthesized.
Interaction in vitro; protein produced as a recombinant fusion proteins in bacterial system or chemically synthesized.
deletion of C-terminal ERLI, coordinates relative to crb-PD
Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from transfected S2R+ cell extract.
