FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
Interacting Genes
FlyBase ID
FBig0000108218
Interaction Network
Interactions Browser links
Dlish network
ft network
Reported Interactions
FBrf0233684-11.coIP.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
ft 

Fat

bait

FLAG tag

ft 

Dlish

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
ft 
intracellular domain
sufficient binding region
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2R+ cell line; bait produced from transfected construct; prey produced from transfected construct.

FBrf0235872-3.coIP.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note

Dlish

bait

RFP tag

ft 

ft

prey

V5 tag

ft 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
ft 
intracellular domain
sufficient binding region
aa 4610-5147
SH3-1 domain
sufficient binding region
aa 61-111
SH3-3 domain
sufficient binding region
aa 293-346
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

FBrf0256335-7.coIP.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note

Ds

bait

FLAG tag

ft 

Ft

prey
ft 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
intracellular domain
sufficient binding region
aa 3115-3556

coordinates relative to Dlish-PA

ft 
intracellular domain
sufficient binding region
unspecified

ECD domain deleted; coordinates relative to ft-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2R+ cell line; bait produced from transfected construct; prey produced from transfected construct.

FBrf0256335-8.PD.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
ft 

Ft

bait

GST tag

ft 

Dlish

prey

FLAG tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
ft 
intracellular domain
sufficient binding region
unspecified

ECD domain deleted; coordinates relative to ft-PA

intracellular domain
sufficient binding region
aa 3115-3556

coordinates relative to Dlish-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

FBrf0260621-7.CL.FM
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
ft 

ft

bait

FLAG tag; fused to cell adhesion protein

ft 

Dlish

prey

HA tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
ft 
intracellular domain
sufficient binding region
aa 1-4920aa 4916-5147

coordinates relative to ft-PA

coordinates relative to ft-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was fixed S2 cells; proteins produced from transfected constructs.

Bait protein was fused to the ed cell adhesion protein, which targets the chimera to cell to cell contacts. The ability of this chimera to recruit prey protein to cell contact sites taken as evidence of direct physical interaction.

External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (4)