me31B, Patr-1 Physical Interaction report

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

me31B

Me31B

bait

HA tag

me31B

Patr-1

HPat

prey

GFP tag

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

me31B

C-terminal RecA-like domain

sufficient binding region

aa 268-459

coordinates relative to me31B-PA

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Haas et al., 2010)

Addition of purified Edc3 FDF motif peptide disrupts the me31B-Patr-1 interaction.

(Haas et al., 2010)

FBrf0210604-8.coIP.WB

Reference

Haas et al., 2010

Description

physical association

Assay

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

Patr-1

HPat

bait

GFP or HA tag

Patr-1

me31B

Me31B

prey

HA or GFP tag

me31B

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Patr-1

N-terminal domain

sufficient binding region

aa 1-56

Patr-1

N-terminal domain

necessary binding region

aa 1-56

me31B

C-terminal RecA-like domain

sufficient binding region

aa 268-459

coordinates relative to me31B-PA

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Haas et al., 2010)

FBrf0212181-3.coIP.WB

Reference

Jäger and Dorner, 2010

Description

physical association

Assay

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

Patr-1

bait

HA tag

me31B

prey

Myc tag

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Jäger and Dorner, 2010)

FBrf0234688-11.coIP.WB

Reference

Sgromo et al., 2017

Description

physical association

Assay

Collection

Source/Stage

Cell line used

S2-ATCC

Participants

Corresponds to

Reported as

Role

Note

Patr-1

HPat

bait

GFP tag

me31B

Me31B

prey

HA tag

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Sgromo et al., 2017)

Positive control.

(Sgromo et al., 2017)

FBrf0236770-24.coIP.WB

Reference

Wang et al., 2017

Description

physical association

Assay

western blot

Collection

Source/Stage

Cell line used

S2-ThermoFischer

Participants

Corresponds to

Reported as

Role

Note

me31B

ME31B

bait

GFP tag

Patr-1

HPat

prey

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Wang et al., 2017)

FBrf0244109-6.coIP.WB

Reference

Peter et al., 2019

Description

physical association

Assay

western blot

Collection

Source/Stage

Cell line used