FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
eIF4A, aub
FlyBase ID
FBig0000109563
Interaction Network
Interactions Browser links
eIF4A network
aub network
Reported Interactions
FBrf0234826-10.coIP.MS
Reference
Zheng et al., 2016
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage 1embryonic stage 2
Cell line used
Participants
Corresponds to
Reported as
Role
Note
aub 

Aub

bait

GFP tag

eIF4A 

eIF4A

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Zheng et al., 2016)

Embryos were formaldehyde crosslinked before affinity purification.

(Zheng et al., 2016)
FBrf0234826-18.coIP.WB
Reference
Zheng et al., 2016
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
aub 

Aub

bait

FLAG tag

eIF4A 

eIF4A

prey

Myc tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait purified from transfected S2 cell extract; prey purified from baculovirus-infected Sf9 cell extract.

(Zheng et al., 2016)
FBrf0235333-8a.coIP.WB
Reference
Ma et al., 2017
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DRSC
Participants
Corresponds to
Reported as
Role
Note
eIF4A 

eIF4A

bait

FLAG tag

aub 

aub

prey

HA tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Ma et al., 2017)
FBrf0235333-9a.coIP.WB
Reference
Ma et al., 2017
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DRSC
Participants
Corresponds to
Reported as
Role
Note
aub 

aub

bait

FLAG tag

eIF4A 

eIF4A

prey

Myc tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Ma et al., 2017)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (2)