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General Information
Name
Furlong_ChIP-chip
Species
D. melanogaster
Project type
FlyBase ID
FBlc0003340
Parent Project
    Data Provider
    Title
    ChIP-chip identification of binding sites for transcription factors that regulate mesodermal development.
    Accessions
      Overview
      Description
      Project attributes
      Reagent type
      Key genes
      (bait_protein)
      GO term(s)
      SO term(s)
      Details
      Sample preparation
      Protocol

      Chromatin was prepared from formaldehyde-fixed embryos as previously described (FBrf0202481).

      Mode of Assay

      Immunoprecipitated chromatin was analyzed by Affymetrix GeneChip Drosophila Tiling 1.0R array

      Data analysis

      Peaks were called with TileMap software (v1) after quantile normalization. For each enriched region, probe intensity peaks were identified as extrema on a smoothed curve of the log2-ratio signal. Finally, 200 bp regions centered on identified extremas were defined. Peak calls were lifted over from Dmel_Release_5 to Dmel_Release_6 using the NCBI Genome Remapping tool.

      Comments
      Additional Information
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      Furlong_ChIP-chip
      Name Synonyms
      ChIP-chip identification of binding sites for transcription factors that regulate mesodermal development.
      Secondary FlyBase IDs
        References (6)