Subject: Revised FB entry for Tpl --========================_64989128==_ Dear Colleagues, I have attached a revised version of the FB entry for Tpl. The revisions consist of correction of errors that date back to the DIS version of Lindsley and Zimm, as well as corrections from new data recently published (Dorer et al, Dec. 1995, Genetics 141(3): 1037-1042. The revision was constructed by downloading the current entry as text, and then editing it. I have noted lines that need to be added or changed from the current entry with a \*, lines that need to be deleted with a >, and lines that consist of my comments (not to be included in the entry, but to explain to you why a change is necessary) with a ~. If you have any questions or if my mailer does not attach the file properly to this message please let me know. Sincerely, Alan Christensen --========================_64989128==_ Content-Disposition: attachment; filename='Tpl' ~Revised FB entry for Tpl. ~Please note: ~Lines beginning with a \* indicate additions or changes to the current entry ~Lines beginning with a ~ indicate comments from me that explain why somethi= ng ~should be changed or deleted ~Lines beginning with a > indicate lines in the current entry that should ~be deleted Gene symbol Tpl \*Full name Triplo-lethal =46lyBase ID number FBgn0003739 Graphic map Synonym(s) tpl Date 13 Oct 95 \*Genetic map position very close to Ki because deletions of Tpl \* also delete Ki (Roehrdanz and Lucchesi, 1980), \* and because no deletions of Ki were obtained \* presumably due to the close proximity of Tpl \* (Kaufman, 1978) Cytological map position Located in 83D5-E1 because included in Df(3R)Tpl4 =3D Df(3R)83D4-5;83E1-2 and Df(3R)Tpl7 =3D Df(3R)83D4-5;83E1-2 (Keppy and Denell, 1979). Revised cyto. map posn. 83E1--83E2 Phenotypic information Unique dosage-sensitive locus at 83D-E; first detected by segmental aneuploidy, using the Y-autosome translocations T(Y;3)L132 and Tp(Y;3)A109 (Lindsley, Sandler, Baker, Carpenter, Denell, Hall, Jacobs, Miklos, Davis, Gethmann, Hardy, Hessler, Miller, Nozawa, Parry and Gould-Somero, 1972). Lethal when present in either one (Tpl/Df) or three doses (Tpl/Dp) in an otherwise diploid individual. These individuals do not survive to the adult stage, but a few larvae with three doses of 83D-E develop to the third instar. The surviving larvae are also hyperploid for the X chromosome (as in 3X;2A metafemales); they can be produced in genotypes duplicated for 7C and 7D-E \* (Roehrdanz and Lucchesi, 1979; Roehrdanz and Lucchesi 1981). This \* interaction is due to the Isis locus (Dorer et al., 1993). Flies \* with a deficiency for 83D-E in one chromosome 3 and a duplication for the region in the other (two doses in all) are viable (Denell, \* 1976). Crosses of these Df/Dp flies to wild type \* fail to produce viable adults (Keppy and Denell, 1979). \* When wild-type flies \* are treated with EMS or gamma-rays (Keppy and Denell, 1979; Roehrdanz and \* Lucchesi, 1980), new deficiencies of Tpl and mutations in the tightly li= nked \* Su(Tpl) locus are obtained (Dorer et al., 1995). No function in measurin= g \* the X/A ratio has been observed in Tpl; Tpl-aneuploids are not rescued by mutations in \* the sex-determining genes Sxl and da (Christensen and Lucchesi, 1988). ~Delete the entire paragraph of Miscellaneous info, as it is incorrect >Miscellaneous info... Data from ref. FBrf0051978 Phenotypic information \* Hybrid dysgenesis has been used to mutate the Tpl locus, but no amorph= ic \* alleles were generated, suggesting Tpl may be a complex locus. ~Delete this sentence because it really refers to Su(Tpl) > A higher mutation rate with hybrid dysgenesis than with > radiation or chemicals suggests a peculiar genetic > organization of Tpl. Data from ref. FBrf0058587 Phenotypic information \* Increased dosage of the Is locus suppresses the triplo-lethal, but \* not the haplo-lethal phenotype of Tpl. Altered dosage of Is in the presence of two copies of Tpl has no obvious effects. Is probably does not directly repress Tpl expression, but rather acts downstream of the Tpl triplo-lethal phenotype. Data from ref. FBrf0066351 Phenotypic information Flies with three or one dose of Tpl die as late embryos or early first instar larvae. Lethality can be rescued by dosage of the \* Is locus, or mutations of the Su(Tpl) locus ~Delete this allele, because it is an allele of Su(Tpl), listed in that entr= y >Allele TplJ34 > FlyBase ID number FBal0030331 > > References > Dorer and Christensen, 1990, Genetics 125: 795--801 > <up>FBrf0051978</up> > > Data from ref. FBrf0051978 > Mutagen PM hybrid dysgenesis > Associated aberration(s) P{}TplJ34 > Phenotypic information Allele class: hypomorph ~Rename because this is NOT a revertant. That was an error in Lindsley and ~Zimm. I suggest keeping the name 10d77-6. It is most likely to have been ~a small deficiency,but it has unfortunately been lost, so we'll never know. \*Allele Tpl10d77-6 Synonym(s) 10d77-6 FlyBase ID number FBal0016998 Mutagen ethyl methanesulfonate References Keppy and Denell, 1979, Genetics 91: 421--441 <up>FBrf0033180</up> ~Rename because this is NOT a revertant. That was an error in Lindsley and ~Zimm. I suggest keeping the name 18i77. It may be a small deficiency, ~so it will probably need to be renamed again, but it's better to wait until ~we know what it really is. \*Allele Tpl18i77 Synonym(s) 18i77 FlyBase ID number FBal0016999 Mutagen formaldehyde References Keppy and Denell, 1979, Genetics 91: 421--441 <up>FBrf0033180</up> Dorer et al., 1993, Genetics 134(1): 243--249 <up>FBrf0058587</up> ~Delete this, and wait until we know what it really is > Data from ref. FBrf0058587 > Synonym(s) Df(3R)l8i77 ~Delete this allele for two reasons: (1) because it is an allele of Su(Tpl), ~listed in that entry, and (2) because it is NOT a revertant - that was an ~error in Lindsley and Zimm, and the renaming of it as Tplrv3 was ~inappropriate and incorrect. >Allele Tplrv3 > Synonym(s) tpl10 > FlyBase ID number FBal0017000 > Mutagen ethyl methanesulfonate > > References > Roehrdanz and Lucchesi, 1980, Genetics 95(2): 355--366 > <up>FBrf0034851</up> > Dorer and Christensen, 1989, Genetics 122: 397--401 > <up>FBrf0049901</up> > Dorer and Christensen, 1990, Genetics 125: 795--801 > <up>FBrf0051978</up> > > Data from ref. FBrf0051978 > Molecular biology data > Insertion of an unidentified mobile element. > Mutagen insertion > Phenotypic information > Allele class: hypomorph > Phenotypic class: lethal | recessive > Site specific increase in recombination frequency and premeiotic > recombination in males. > Synonym(s) Tpl10 ~Delete this allele for two reasons: (1) because it is an allele of Su(Tpl), ~listed in that entry, and (2) because it is NOT a revertant - that was an ~error in Lindsley and Zimm, and the renaming of it as Tplrv4 was ~inappropriate and incorrect. >Allele Tplrv4 > Synonym(s) tpl17 > FlyBase ID number FBal0017001 > Mutagen ethyl methanesulfonate > Phenotypic information > Causes an increase in recombination between flanking markers > of 6.5-10.5 times, while recombination in other adjacent > regions is not changed. > > References > Roehrdanz and Lucchesi, 1980, Genetics 95(2): 355--366 > <up>FBrf0034851</up> > Dorer and Christensen, 1989, Genetics 122: 397--401 > <up>FBrf0049901</up> > Dorer and Christensen, 1990, Genetics 125: 795--801 > <up>FBrf0051978</up> > > Data from ref. FBrf0051978 > Synonym(s) Tpl17 > Molecular biology data > Insertion of an unidentified mobile element. > Mutagen insertion > Phenotypic information > Allele class: hypomorph > Site specific increase in recombination frequency and premeiotic > recombination in males. ~Delete this allele for two reasons: (1) because it is an allele of Su(Tpl), ~listed in that entry, and (2) because it is NOT a revertant - that was an ~error in Lindsley and Zimm, and the renaming of it as Tplrv5 was ~inappropriate and incorrect. >Allele Tplrv5 > Synonym(s) tpl38 > FlyBase ID number FBal0017002 > Mutagen ethyl methanesulfonate > Phenotypic information > Causes an increase in recombination between flanking markers > of 6.5-10.5 times, while recombination in other adjacent > regions is not changed. > > References > Roehrdanz andLucchesi, 1980, Genetics 95(2): 355--366 > <up>FBrf0034851</up> > Dorer and Christensen, 1989, Genetics 122: 397--401 > <up>FBrf0049901</up> > Dorer and Christensen, 1990, Genetics 125: 795--801 > <up>FBrf0051978</up> > > Data from ref. FBrf0051978 > Synonym(s) Tpl38 > Molecular biology data > Insertion of an unidentified mobile element. > Mutagen insertion > Phenotypic information > Allele class: hypomorph > Site specific increase in recombination frequency and premeiotic > recombination in males. References Lindsley et al., 1972, Genetics 71: 157--184 <up>FBrf0023910</up> Denell, 1974, D. I. S. 51: 124 <up>FBrf0026052</up> Denell, 1976, Genetics 84: 193--210 <up>FBrf0028747</up> \* Kaufman, 1978, Genetics 90(3): 579--596 Fbrf0031649 Keppy and Denell, 1979, Genetics 91: 421--441 <up>FBrf0033180</up> Roehrdanz and Lucchesi, 1979, Genetics 91(4/2): s105 <up>FBrf0033226</up> Lucchesi and Roehrdanz, 1979, Genetics 91(4/2): s71 <up>FBrf0033295</up> Roehrdanz and Lucchesi, 1980, Genetics 95(2): 355--366 <up>FBrf0034851</up> Roehrdanz and Lucchesi, 1981, Dev. Genet. 2(2): 147--158 <up>FBrf0036224</up> Kennison and Russell, 1987, Genetics 116: 75--86 <up>FBrf0046404</up> Christensen and Lucchesi, 1988, D. I. S. 67: 15 <up>FBrf0047737</up> Dorer and Christensen, 1989, Genetics 122: 397--401 <up>FBrf0049901</up> Dorer and Christensen, 1990, Genetics 125: 795--801 <up>FBrf0051978</up> Dorer et al., 1990, Nucleic Acids Res. 18: 5489--5494 <up>FBrf0052735</up> Dorer et al., 1993, Genetics 134(1): 243--249 <up>FBrf0058587</up> Christensen et al., 1993, Int. Congr. Genet. 17 Abstr.: 179 <up>FBrf0058726</up> Christensen and Dorer, 1989, A. Conf. Dros. Res. 30: 15 <up>FBrf0066351</up> Lindsley and Zimm, 1992 <up>FBrf0066905</up> Barry and Christensen, 1994, A. Conf. Dros. Res. 35: 319 <up>FBrf0068319</up> Christensen et al., 1995, A. Conf. Dros. Res. 36 Suppl.: 286A Fbrf0078212 \* Dorer et al., 1995, Genetics 141(3): 1037-1042 --========================_64989128==_ --========================_64989128==_-- Subject: Su(Tpl): revised FB entry --========================_64989128==_ Dear Colleagues, I have attached a revised version of the FB entry for Su(Tpl). This accompanies my previous update of Tpl. The symbols I have used for comments, additions or deletions are as described. If you have any questions or if my mailer does not attach the file properly to this message please let me know. Sincerely, Alan Christensen --========================_64989128==_ Content-Disposition: attachment; filename='Su(Tpl)' ~Revised FB entry for Su(Tpl). ~Please note: ~Lines beginning with a \* indicate additions or changes to the current entry ~Lines beginning with a ~ indicate comments from me that explain why somethi= ng ~should be changed or deleted ~Lines beginning with a > indicate lines in the current entry that should ~be deleted Gene symbol Su(Tpl) =46lyBase ID number FBgn0014037 Date 13 Oct 95 \*Genetic map position 3-46.5 (Dorer et al., 1995); \* between st and ri. Cytological map position \* Located in 76B-D because included in Df(3L)kto2 (Dorer et al., 1995) ~A better reference for this information would be Dorer et al, 1995 ~which I do not know the Fbrf# of (see below) Data from ref. FBrf0058587 Phenotypic information Mutations at Su(Tpl) block the triplo-lethal effect of Tpl. Several alleles previously thought to be hypomorphic mutations of Tpl are dominant mutations of the tightly linked Su(Tpl). \* Su(Tpl) mutations are also recessive lethal. Allele Su(Tpl)10 Synonym(s) Tpl10 Tplrv3 FlyBase ID number FBal0034034 \* Mutagen ethyl methanesulfonate \* \* References \* Roehrdanz and Lucchesi, 1980, Genetics 95(2): 355--366 \* <up>FBrf0034851</up> \* Dorer and Christensen, 1989, Genetics 122: 397--401 \* <up>FBrf0049901</up> \* Dorer and Christensen, 1990, Genetics 125: 795--801 \* <up>FBrf0051978</up> \* Dorer et al., 1995, Genetics 141(3): 1037-1042 \* FBrf??????? \* Data from ref. FBrf0051978 \* Molecular biology data \* Possible insertion of an unidentified mobile element. \* Phenotypic information \* Site-specific increase in recombination frequency and premeiotic \* recombination in males. \*Allele Su(Tpl)17 \* Synonym(s) Tpl17 \* Tplrv4 \* FlyBase ID number FBal??????? \* Mutagen ethyl methanesulfonate \* \* References \* Roehrdanz and Lucchesi, 1980, Genetics 95(2): 355--366 \* <up>FBrf0034851</up> \* Dorer and Christensen, 1989, Genetics 122: 397--401 \* <up>FBrf0049901</up> \* Dorer and Christensen, 1990, Genetics 125: 795--801 \* <up>FBrf0051978</up> \* Dorer et al., 1995, Genetics 141(3): 1037-1042 \* FBrf??????? \* Data from ref. FBrf0051978 \* Molecular biology data \* Possible insertion of an unidentified mobile element. \* Phenotypic information \* Site-specific increase in recombination frequency and premeiotic \* recombination in males. \*Allele Su(Tpl)38 \* Synonym(s) Tpl38 \* Tplrv5 \* FlyBase ID number FBal??????? \* Mutagen ethyl methanesulfonate \* \* References \* Roehrdanz and Lucchesi, 1980, Genetics 95(2): 355--366 \* <up>FBrf0034851</up> \* Dorer and Christensen, 1989, Genetics 122: 397--401 \* <up>FBrf0049901</up> \* Dorer and Christensen, 1990, Genetics 125: 795--801 \* <up>FBrf0051978</up> ~I don't know the Fbrf# of this, or if it has one yet \* Dorer et al., 1995, Genetics 141(3): 1037-1042 \* FBrf??????? \* Data from ref. FBrf0051978 \* Molecular biology data \* Possible insertion of an unidentified mobile element. \* Phenotypic information \* Site-specific increase in recombination frequency and premeiotic \* recombination in males. \*Allele Su(Tpl)J34 \* Synonym(s) TplJ34 \* FlyBase ID number FBal??????? \* Data from ref. Fbrf0058587 and Dorer at al., 1995 Phenotypic information \* Phenotypic class: suppressor | dominant, lethal | recessive Suppresses the triplo-lethal effect of Tpl. \* Data from ref. FBrf0051978 \* Mutagen PM hybrid dysgenesis \* Associated aberration(s) P{}TplJ34 References Dorer et al., 1993, Genetics 134(1): 243--249 Fbrf0058587 ~I don't know the Fbrf# of this, or if it has one yet \* Dorer et al., 1995, Genetics 141(3): 1037-1042FBrf??????? --========================_64989128==_--
