Dear Chihiro, .. So here are my answers to all of your questions: pUAST-pro-dronc: Complete coding sequence from nt 369 to1721 pUAST-(Delta)N dronc: Dronc without its prodomain; coding sequence from nt 706 to1721. pUAST-pro-dronc C->A: Complete coding sequence of dronc (from nt 369 to1721) but with the catalytic Cysteine mutated to Alanine (Aa substitution). This protein is a catalytically inactive version of dronc and does not induce cell death. pUAST-(Delta)N dronc C->A: Dronc without its prodomain; coding sequence from nt 706 to1721 but with the catalytic Cysteine mutated to Alanine (Aa substitution). This protein is a catalytically inactive version of dronc and does not induce cell death. pUAST-dronc-CARD: Prodomain of dronc on its own; coding sequence form nt 369 to 786. .. Yours sincerely Pascal Dr. Pascal Meier Biochemistry of the Cell Nucleus Laboratory Imperial Cancer Research Fund 44 Lincoln's Inn Fields London WC2A 3PX ================================================================================ Dear Dr Evan, I am currently curating your paper for FlyBase: Meier at al., 2000 EMBO J. 19:598--611 . I have a few questions I was hoping you could answer for me. \--DRONC constructs You use a number of DRONC constructs which you insert into pUAST and transform into flies. Could you give me some more details on the molecular details of these constructs? e.g. Is it a full length construct, what fragment of the cDNA was used (or has been deleted) in each construct? You call them: pro-dronc (Delta)dronc pro-dronc C->A (Delta)dronc C->A dronc-card I hope to hear from you soon, Best wishes, Chihiro \---------------------------------------------------------------------- Chihiro Yamada. FlyBase (Cambridge), \----------------------------------------------------------------------
