FlyBase Allele Report: Dmel\Dronc[ΔN.C-A.UAS]

General Information

Symbol

Dmel\Dronc^ΔN.C-A.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0104750

Feature type

allele

Associated gene

Dmel\Dronc

Associated Insertion(s)

Carried in Construct

P{UAS-ΔNdronc.C->A}

Key Links

Transgenic product class

inframe_deletion

(Meier, 2000.4.5)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

inframe_deletion

(Meier, 2000.4.5)

Mutagen

in vitro construct

(Meier, 2000.4.5)

Progenitor genotype

Carried in construct

P{UAS-ΔNdronc.C->A}

Cytology

Description

A catalytically inactive and truncated coding sequence of Dronc is expressed under the control of UASt sequences. Includes nucleotides 706 to 1721, missing its prodomain and with its catalytic Cys mutated to Ala.

(Meier, 2000.4.5)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Dronc

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy, with Scer\GAL4^ppk.1.9

(Williams et al., 2006)

lethal - all die before end of pupal stage, with Scer\GAL4^Lsp2.PH

(Liu et al., 2013)

some die during pupal stage, with Scer\GAL4^Lsp2.PH

(Liu et al., 2013)

Phenotype Manifest In

dendrite, with Scer\GAL4^ppk.1.9

(Williams et al., 2006)

larval dorsal multidendritic neuron ddaC, with Scer\GAL4^ppk.1.9

(Williams et al., 2006)

Detailed Description

Statement

Reference

Expression of Nc^{ΔN.C-A.Scer\UAS} under the control of Scer\GAL4^Lsp2.PH results in 25% pupal lethality.

(Liu et al., 2013)

Overexpression of Nc^{ΔN.C-A.Scer\UAS} in the ddaC neurons under the control of Scer\GAL4^ppk.1.9 results in a marked suppression of dendritic pruning. At 14 hours after pupal formation, the dendrites are detached from the soma but still largely cover the same 'footprint' as in third instar larvae. The morphology of detached branches varies: some show proximal swellings, whereas others are thinned and beaded along their entire length.

(Williams et al., 2006)

Expression of Nc^{ΔN.C-A.Scer\UAS} in the eye under the control of Scer\GAL4^GMR.PF gave no visible phenotype.

(Meier et al., 2000)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Dronc^ΔN.C-A.UAS, Scer\GAL4^Lsp2.PH has some die during pupal stage phenotype, enhanceable by Atg1^K38Q.UAS, Scer\GAL4^Lsp2.PH

(Liu et al., 2013)

Dronc^ΔN.C-A.UAS, Scer\GAL4^Lsp2.PH has lethal - all die before end of pupal stage phenotype, enhanceable by Dronc^ΔN.C-A.UAS, Scer\GAL4^Lsp2.PH

(Liu et al., 2013)

Enhancer of

Statement

Reference

Dronc^ΔN.C-A.UAS, Scer\GAL4^Lsp2.PH is an enhancer of some die during pupal stage phenotype of Atg1^K38Q.UAS, Scer\GAL4^Lsp2.PH

(Liu et al., 2013)

Dronc^ΔN.C-A.UAS, Scer\GAL4^Lsp2.PH is an enhancer of lethal - all die before end of pupal stage phenotype of Atg1^K38Q.UAS, Scer\GAL4^Lsp2.PH

(Liu et al., 2013)

Dronc^ΔN.C-A.UAS, Scer\GAL4^Lsp2.PH is an enhancer of lethal - all die before end of pupal stage phenotype of Dronc^ΔN.C-A.UAS, Scer\GAL4^Lsp2.PH

(Liu et al., 2013)

NOT Suppressor of

Statement

Reference

Scer\GAL4^ey-OK107/Dronc^ΔN.C-A.UAS is a non-suppressor of abnormal neuroanatomy | somatic clone | adult stage | progressive phenotype of bsk^flp147E

(Rallis et al., 2013)

Other

Statement

Reference

Atg1^K38Q.UAS, Dronc^ΔN.C-A.UAS, Scer\GAL4^Lsp2.PH has abnormal developmental rate phenotype

(Liu et al., 2013)

Atg1^K38Q.UAS, Dronc^ΔN.C-A.UAS, Scer\GAL4^Lsp2.PH has decreased cell death phenotype

(Liu et al., 2013)

Phenotype Manifest In

Suppressor of

Statement

Reference

Dronc^ΔN.C-A.UAS, Scer\GAL4^slbo.2.6 is a suppressor | partially of border follicle cell phenotype of Rac1^N17.UAS, Scer\GAL4^slbo.2.6

(Geisbrecht and Montell, 2004)

NOT Suppressor of

Statement

Reference

Scer\GAL4^ey-OK107/Dronc^ΔN.C-A.UAS is a non-suppressor of alpha/beta Kenyon cell phenotype of bsk^flp147E

(Rallis et al., 2013)

Scer\GAL4^ey-OK107/Dronc^ΔN.C-A.UAS is a non-suppressor of gamma Kenyon cell phenotype of bsk^flp147E

(Rallis et al., 2013)

Other

Statement

Reference

Dronc^ΔN.C-A.UAS, Scer\GAL4^Lsp2.PH has fat body phenotype

(Liu et al., 2013)

Atg1^K38Q.UAS, Dronc^ΔN.C-A.UAS, Scer\GAL4^Lsp2.PH has embryonic/larval fat body phenotype

(Liu et al., 2013)

Atg1^K38Q.UAS, Dronc^ΔN.C-A.UAS, Scer\GAL4^Lsp2.PH has fat body phenotype

(Liu et al., 2013)

Additional Comments

Genetic Interactions

Statement

Reference

Simultaneous overexpression of Atg1^{K38Q.Scer\UAS} and Nc^{ΔN.C-A.Scer\UAS} in the remodelling fat body, under the control of Scer\GAL4^Lsp2.PH dramatically reduces autophagy and cell death but increases lethality by approximately 40%.

Simultaneous overexpression of Atg1^{K38Q.Scer\UAS} and Nc^{ΔN.C-A.Scer\UAS} in the remodelling fat body, under the control of Scer\GAL4^Lsp2.PH delays pupal development by approximately 4 hours.

(Liu et al., 2013)

Expression of two copies of Nc^{ΔN.C-A.Scer\UAS} under the control of Scer\GAL4^ey-OK107 fails to suppress the axon degeneration seen in bsk^flp147E mutant mushroom body neuron clones.

(Rallis et al., 2013)

The border follicle cell migration defect seen in egg chambers expressing Rac1^N17.Scer\UAS under the control of Scer\GAL4^slbo.2.6 is partially suppressed by coexpression of Nc^{ΔN.C-A.Scer\UAS}.

(Geisbrecht and Montell, 2004)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

Dronc^ΔN.C-A.UAS rescues Dronc^I29/Dronc^L32

(Napoletano et al., 2017)

Comments

The expression of Dronc^{ΔN.C-A.Scer\UAS} under the control of Scer\GAL4^nos.PU rescues both the significant proportion of hyperplastic adult testes and the increased necrosis at the apical tip of testes observed in Dronc^I29/Dronc^L32 transheterozygotes.

(Napoletano et al., 2017)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Dronc^{ΔN.C-A.Scer\UAS}

Dronc^ΔN.C-A.UAS

Nc^{ΔN.C-A.Scer\UAS}

Name Synonyms

Secondary FlyBase IDs

References (9)

Report Sections

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