Open Close
General Information
Symbol
Dmel\DroncL32
Species
D. melanogaster
Name
FlyBase ID
FBal0189653
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Key Links
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Nucleotide change:

T9968961A

Amino acid change:

L25Q | Dronc-PA

Reported amino acid change:

L32E

Comment:

The leucine at 25 is in fact mutated to a glutamine, rather than a glutamate residue.

Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

Nucleotide substitution: T?A.

Amino acid replacement: L24Q.

Amino acid replacement: L25E.

Amino acid replacement: L25Q.

FlyBase curator comment: the leucine at 25 is in fact mutated to a glutamine, rather than a glutamate residue.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 1 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

DroncI29/DroncL32 transheterozygotes exhibit a significant increase in the proportion of hyperplastic testes as compared to controls; these testes exhibit a significant decrease in necrosis at their apical tip (as shown by the number of spermatogonial cysts bearing Propidium Iodine-positive or TUNEL-positive cells), as compared to controls. During spermatogenesis, cystic bulges and waste bags are largely absent and actin investment cones in the individualization complex are uncoordinated, as compared to controls.

NcI24/NcL32 mutant males exhibit ~40% less spermatogonial cyst death compared to controls.

NcI29/NcL32 mutant males exhibit ~60% less spermatogonial cyst death compared to controls.

NcL32 mutants display severe defects during the spermatid individualization process. The cystic bulges are frequently reduced in size or appear flat due to a failure in the appropriate collection of the cytoplasm of the spermatids . The retained cytoplasm is clearly visualized as a trail along the entire length of what was supposed to have been the post-individualized portion of the spermatids. Frequently a large portion of the spermatid cytoplasm is retained in a 'mini' cystic bulge structure, which often contains part of the individualization complex. Waste bags are also reduced in size.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressor of
Statement
Reference
Phenotype Manifest In
Suppressor of
Statement
Reference

DroncI24/DroncL32 is a suppressor | partially of ovariole phenotype of Diap16B/Diap18

DroncL32/DroncL32 is a suppressor of eye | somatic clone phenotype of rprGMR.PW

Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Partially rescued by
Not rescued by
Comments

Both the significant proportion of hyperplastic adult testes and the increased necrosis at the apical tip of the testes observed in DroncI29/DroncL32 transheterozygotes are rescued by the expression of Droncpro.Scer\UAS, Droncpro.C-A.Scer\UAS, DroncΔN.Scer\UAS, or DroncΔN.C-A.Scer\UAS, but not DroncCARD.Scer\UAS, under the control of Scer\GAL4nos.PU. The significant proportion of hyperplastic adult testes is also rescued by the expression of either DroncWT.5'3'UTR or DroncC-A.5'3'UTR.

The sperm individualization defects observed in DroncI29/DroncL32 transheterozygotes are not rescued by the expression of either DroncWT.5'3'UTR or DroncC-A.5'3'UTR.

Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer

Selected as: a recessive suppressor of the WGMR.PG eye ablation phenotype.

Selected as: a mutant that recessively suppresses the eye ablation phenotype caused by eye-specific overexpression of W.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Name Synonyms
Secondary FlyBase IDs
    References (7)