The following information accompanied stocks donated to the Bloomington Stock Center by Akira Goto, Nagoya University (11/01).
The identification and characterization of Hml was described in Goto et al. 2001, Biochem J. 359: 99-108 (FBrf0138238).
pP{Hml-GAL4.G} was constructed by combining a GAL4-spanning BamHI-NotI fragment excised from pGaTB and the 3.0 kb upstream promoter region of Hml and cloning them into pP{CaSpeR-4}. The promoter region of Hml flanked by BamHI sites was prepared by amplification of genomic DNA using primers
5'-CGCGGATCCAAGAACTTTACATAAATTCCGGTATC-3' and 5'-CGCGGATCCTTTGTTAGGCTAATCGGAAATTGTTGG-3' based on the sequence nt
20501-23450 in GenBank AC015143.
Insertions of P{Hml-GAL4.G} include the following:
P{Hml-GAL4.G}5-6 homozygous viable and fertile chromosome 3 insertion
P{Hml-GAL4.G}6-4 homozygous viable and fertile chromosome 2 insertion
P{Hml-GAL4.G}8-5 homozygous viable and fertile chromosome 3 insertion
P{Hml-GAL4.G}13-6 homozygous viable and fertile chromosome 3 insertion
Endogenous Hml is expressed at low levels in larval hemocytes.
A second chromosome with P{Hml-GAL4.G}6-4, P{UAS- GFP::lacZ.nls } and P{UAS-GFP.S65T} insertions gave weak but detectable GFP expression when heterozygous, and strong GFP expression when homozygous. GFP expression was not detectable when a P{Hml-GAL4.G} insertion was crossed to single UAS-GFP construct insertions.