FlyBase Recombinant Construct Report: P{Hml-GAL4.G}

General Information

Symbol

P{Hml-GAL4.G}

FlyBase ID

FBtp0015155

Feature type

transgenic_transposable_element

Component Allele(s)

Scer\GAL4^Hml.PG

Tsp68C^+t3.0

w^+mC

Also Known As

Hml-GAL4, Hemolectin-GAL4

Size

Expression Data

GAL4 reporter/driver

Associated insertion(s)

4 available

Molecular map

Description and Uses

Location-dependent role

Description

CV term

Qualifiers and info

Reference

transposon

P-element

(Sinenko and Mathey-Prevot, 2004, Goto, 2001.12.6)

Construct components

Product class / Tool use(s)

Marker(s)

w^+mC

Component allele Scer\GAL4^Hml.PG

Product class / Tool use(s)

Regulatory region(s)

Hml

Encoded product / tool

GAL4

binary expression system - driver

Description

The 3.0kb upstream promoter region of Hml regulates expression of a GAL4 driver.

(Goto et al., 2003, Goto, 2001.12.6)

The P{Hml-GAL4.G} construct contains sequences from upstream of Hml driving GAL4 expression (represented by the Scer\GAL4^Hml.PG allele). This genomic fragment from upstream of Hml also contains the complete open reading frame of Tsp68C (represented by the Tsp68C^+t3.0 allele).

(Sinenko and Mathey-Prevot, 2004)

Component allele w^+mC

Product class / Tool use(s)

Regulatory region(s)

Description

4.15kb SpeI fragment of the w gene that carries a deletion of a large part of the first intron and the regulatory region is reduced to 300bp preceding the transcription start site.

(Pirrotta, 1988)

Component allele Tsp68C^+t3.0

Product class / Tool use(s)

Regulatory region(s)

Tsp68C

Description

The P{Hml-GAL4.G} construct contains sequences from upstream of Hml driving Scer\GAL4 expression (represented by the Scer\GAL4^Hml.PG allele). This genomic fragment from upstream of Hml also contains the complete open reading frame of Tsp68C (represented by the Tsp68C^+t3.0 allele).

(Sinenko and Mathey-Prevot, 2004)

Sequence Data

Sequence (FB)

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA Sequence

Extent

Segments and Size

Total Size

Left end

Right end

Segments

Num

Orientation

Symbol

Reference

Features

CV term

Qualifiers and info

Reference

complete

rescue

(Sinenko and Mathey-Prevot, 2004)

selectable

marker

(Goto, 2001.12.6)

Component Alleles

Allele

Scer\GAL4^Hml.PG

Reference(s)

(Iatsenko et al., 2020, Iwashita et al., 2020, Shin et al., 2020, Alpar et al., 2018, Giordani et al., 2016, Grillet et al., 2016, Li and Dijkers, 2015, Liu et al., 2015, Merkling et al., 2015, Arefin et al., 2014, Ashton-Beaucage et al., 2014, Bonnay et al., 2014, Gueguen et al., 2013, Parisi et al., 2013, Chen et al., 2012, Howell et al., 2012, Jones et al., 2012, Tran et al., 2012, Gafuik and Steller, 2011, Kester and Nambu, 2011, Kugler et al., 2011, Kulkarni et al., 2011, Flaherty et al., 2010, Junell et al., 2010, Mabery and Schneider, 2010, McPhee et al., 2010, Nagai et al., 2010, Paddibhatla et al., 2010, Cronin et al., 2009, Jacques et al., 2009, Shelly et al., 2009, Shia et al., 2009, Bergeret et al., 2008, Lindgren et al., 2008, Schmidt et al., 2008, Venkatachalam et al., 2008, Dijkers and O'Farrell, 2007, Korolchuk et al., 2007, Akdemir et al., 2006, Brun et al., 2006, Scherfer et al., 2006, Serpe and O'Connor, 2006, Dolezal et al., 2005, Jung et al., 2005, Kocks et al., 2005, Watson et al., 2005, Williams et al., 2005, Chew et al., 2004, Crozatier et al., 2004, Narita et al., 2004, Sinenko and Mathey-Prevot, 2004, Yoshida et al., 2004, Zettervall et al., 2004, Agaisse et al., 2003, Goto et al., 2003, Goto, 2001.12.6)

Molecular data

(Sinenko and Mathey-Prevot, 2004)

The 3.0kb upstream promoter region of Hml regulates expression of a GAL4 driver.

(Goto et al., 2003, Goto, 2001.12.6)

Phenotype Data

Phenotypic Class Phenotype Manifest In Detailed Description

Allele

Tsp68C^+t3.0

Reference(s)

(Sinenko and Mathey-Prevot, 2004)

Molecular data

(Sinenko and Mathey-Prevot, 2004)

Phenotype Data

Phenotypic Class Phenotype Manifest In Detailed Description

Allele

w^+mC

Reference(s)

Molecular data

4.15kb SpeI fragment of the w gene that carries a deletion of a large part of the first intron and the regulatory region is reduced to 300bp preceding the transcription start site.

(Pirrotta, 1988)

Phenotype Data

Phenotypic Class Phenotype Manifest In Detailed Description

Expression Data

Reporter Expression

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 17 -- prepupal stage P1

hemocyte

(Goto et al., 2003)

larval stage

hemocyte

(Narita et al., 2004, Goto, 2001.12.6)

embryonic/larval hemocyte

(Kester and Nambu, 2011)

larval stage -- adult stage

hemocyte

(Shia et al., 2009)

Additional Information

Statement

Reference

Drives expression in hemocytes at late embryonic and all post-embryonic stages.

(FlyBase Curators, 2017)

Scer\GAL4^Hml.PG drives expression in hemocytes at post-embryonic stages.

(Shia et al., 2009)

Marker for

hemocyte

(Narita et al., 2004, Goto et al., 2003, Goto, 2001.12.6)

Reflects expression of

Hml

(Shia et al., 2009, Narita et al., 2004, Goto et al., 2003, Goto, 2001.12.6)

Reporter construct used in assay

Progenitors and Descendants

progenitor(s)

descendant(s)

Comments

Stocks (6)

Bloomington

6395

w¹¹¹⁸; P{Hml-GAL4.G}5-6

6396

w¹¹¹⁸; P{Hml-GAL4.G}6-4

6397

w¹¹¹⁸; P{Hml-GAL4.G}6-4 P{UAS-GFP::lacZ.nls}15.1 P{UAS-GFP.S65T}Myo31DF^T2

Kyoto

108766

w¹¹¹⁸; P{Hml-GAL4.G}5-6

108767

w¹¹¹⁸; P{Hml-GAL4.G}6-4

108768

w¹¹¹⁸; P{Hml-GAL4.G}6-4 P{UAS-GFP::lacZ.nls}15.1 P{UAS-GFP.S65T}Myo31DF^T2

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (23)

Reported As

Symbol Synonym

(hml)-Gal4)

(Liu et al., 2015)

6-4

(Tran et al., 2012)

HML-GAL4

(Cronin et al., 2009, Schmidt et al., 2008)

Hemolectin (hml)-GAL4

(Brun et al., 2006)

Hemolectin GAL4

(Williams et al., 2005)

Hemolectin-GAL4

(Shelly et al., 2009, Zettervall et al., 2004)

Hemolectin-Gal4

(Ashton-Beaucage et al., 2014)

HemolectinGAL4

(Kulkarni et al., 2011)

Hml-GAL4

(Grillet et al., 2016, Howell et al., 2012, Gafuik and Steller, 2011, Junell et al., 2010, Nagai et al., 2010, Watson et al., 2005, Chew et al., 2004)

Hml-Gal4

(Shin et al., 2020, Alpar et al., 2018, Merkling et al., 2015, Bonnay et al., 2014, Gueguen et al., 2013, Flaherty et al., 2010, Paddibhatla et al., 2010, Korolchuk et al., 2007, Akdemir et al., 2006, Dolezal et al., 2005)

Hml-Gal4G

(Kugler et al., 2011)

P(hml-GAL4)

(Agaisse et al., 2003)

P{Hml-GAL4.G}

(Kester and Nambu, 2011, Junell et al., 2010, Korolchuk et al., 2007, FlyBase, 1996-)

hemocyte Gal4

(Dijkers and O'Farrell, 2007)

hemocyte-specific driver

(Akdemir et al., 2006)

hemolectin-GAL4

(Parisi et al., 2013, Shia et al., 2009)

hemolectin-Gal4

(Merkling et al., 2015, Mabery and Schneider, 2010)

hemolectin-gal4

(Giordani et al., 2016, Jacques et al., 2009)

hml-GAL4

(Iatsenko et al., 2020, Iwashita et al., 2020, Arefin et al., 2014, Jones et al., 2012, Shia et al., 2009, Venkatachalam et al., 2008, Scherfer et al., 2006, Narita et al., 2004)

hml-Gal4

(Giordani et al., 2016, Li and Dijkers, 2015, Arefin et al., 2014, McPhee et al., 2010, Serpe and O'Connor, 2006, Kocks et al., 2005, Crozatier et al., 2004, Sinenko and Mathey-Prevot, 2004)

hml-gal4

(Chen et al., 2012, Jung et al., 2005)

hmlGal4

(Bergeret et al., 2008)

pP{hml-GAL4}

(Goto et al., 2003)

Secondary FlyBase IDs

References (57)

Report Sections