Isolation and characterization of Df(3L)BSC12
Jennifer Deal and Kevin Cook
Bloomington Drosophila Stock Center
Df(3L)BSC12 was isolated as a P transposase-induced male recombination event involving P{PZ}l(3)0147001470 and P{EP}capsEP3557. The deletion was isolated as a rhove-1-e1 recombinant chromosome from the cross rhove-1 pp e1 females x CyO, H{PDelta2-3}HoP2.1/+; rhove-1 P{PZ}l(3)0147001470/P{EP}capsEP3557 e1 males. The miniwhite markers from P{lacW} and P{EP} were deleted or disrupted based on the w- eye color of flies carrying the deletion chromosome in the presence of w1.
Df(3L)BSC12 failed to complement P{lacW}trnS064117 and In(3LR)C190.
In polytene squashes of Df(3L)BSC12 heterozygotes, the 70A1,2 doublet appeared attenuated, but not absent. The 70A4,5 doublet and 69F5 were present, but the 69F6, 69F7 and 70A3 bands were not visible in our preparations. Observations detailed below argue that the distal side of 70A1,2 was deleted, so we have denoted the breakpoints of Df(3L)BSC12 as 69F6-70A1;70A1-2.
To reconcile the noncomplementation of Df(3L)BSC12 and the 3L breakpoint of In(3LR)C190, we reexamined the polytene cytology of In(3LR)C190. We found that the 3L breakpoint of In(3LR)C190 lies within the 70A1,2 doublet, splitting off a distal portion of the doublet (likely just the distal edge of 70A1) as a thin, single band. Our revised cytology of In(3LR)C190 is 70A1-2;89D3-6.
Our screens for Df(3L)BSC10 (69D4-5;69F5-7) and Df(3L)BSC12 (69F6-70A1;70A1-2) were designed to isolate deletions that flank M(3)69E as closely as possible. Two closely-linked ribosomal protein genes, RpS12 and RpS4, lie between the predicted endpoints of the two deletions (Adams, M.D. et al. 2000, Science 287: 2185-2195). Our work does not indicate whether M(3)69E corresponds to RpS12 or RpS4, or whether the genetically-defined M(3)69E locus is a composite of both ribosomal protein genes.