The following information accompanied stocks donated to the Bloomington Stock Center by Wouter Ferro, Leiden University Medical College (12/02).
An EMS screen for lethal mutations that fail to complement Df(2L)JS17 was described in Eeken et al., 1997 (Isolation and characterization of two MMS-sensitive mutants in region 23C-23D. A. Dros. Res. Conf. 38: 272A; (FBrf0092383) and in the FlyBase personal communication FBrf0125088.
cn1 bw1 chromosomes were mutagenized and screened for lethality over Df(2L)JS17.
The following numbers of alleles were recovered:
l(2)23Ca 1
l(2)23Cb 3
l(2)23Cc 4
l(2)23Cd 2
l(2)23Ce 4
l(2)23CDa 4
l(2)23CDb 4
l(2)23CDc 3
l(2)23CDd 4
l(2)23CDe 4
l(2)23CDf 2
l(2)23CDg 3
l(2)23Da 5
l(2)23Db 1
toc 34
The following are specific alleles of these complementation groups:
l(2)23CbA20-2
l(2)23CeA14-9
l(2)23CeA15-2
l(2)23CDaA4-3
l(2)23CDaA14-6
l(2)23CDbA3-2
l(2)23CDcA14-4
l(2)23CDdA9-3
l(2)23CDdA19-9
l(2)23CDfA18-2
l(2)23DaA9-4
tocA1-7
l(2)23Cc is allelic to lilliputian based on the noncomplementation of EMS mutations with lillik05431.
Small deletions isolated in this screen gave the following complementation results:
Df(2L)16 failed to complement mutations in l(2)23CDa and l(2)23CDb
Df(2L)17 failed to complement mutations in l(2)23CDc and toc
Df(2L)18 failed to complement mutations in toc, l(2)23CDd and l(2)23CDg
Df(2L)19 failed to complement mutations in l(2)23CDd and l(2)23CDg
Df(2L)20 failed to complement mutations in l(2)23CDe and l(2)23CDf
Df(2L)16 through 20 complemented other mutations from the screen.
Small deletions from other screens gave the following complementation results:
Df(2L)JS25 and Df(2L)JS27 failed to complement mutations in l(2)23CDb,
l(2)23CDc, l(2)23CDd, l(2)23CDe, l(2)23CDf, l(2)23CDg and toc, but complemented other mutations from the screen.
Df(2L)AS2 complemented lilli mutations and failed to complement l(2)23Cd, l(2)23Ce and toc mutations, but other mutations from the screen were not tested.
Df(2L)AS4 failed to complement toc, l(2)23CDd, l(2)23CDf and l(2)23Da mutations, but other mutations from the screen were not tested.
Df(2L)AS9 failed to complement toc, l(2)23CDf and l(2)23Da mutations, but other mutations from the screen were not tested.
Df(2L)AS8 failed to complement mutations in l(2)23Cb, l(2)23Ca and okra, but complemented other mutations from the screen.
Df(2L)C28 failed to complement l(2)23Da and l(2)23Db mutations, but complemented other mutations from the screen.
These results allow the loci to be arranged in the distal-to-proximal order:
l(2)23Cc
l(2)23Cd and l(2)23Ce
l(2)23CDa
l(2)23CDb
l(2)23CDc
toc
l(2)23CDd
l(2)23CDg
l(2)23CDe
l(2)23CDf
l(2)23Da and l(2)23Db
l(2)23Cb, l(2)23Ca and okra
Since these results place l(2)23Ca and l(2)23Cb in 23D, they should be renamed l(2)23Dc and l(2)23Dd, respectively.