Saulière, J., Haque, N., Harms, S., Barbosa, I., Blanchette, M., Le Hir, H. (2010). The exon junction complex differentially marks spliced junctions. Nat. Struct. Mol. Biol. 17(10): 1269--1271.
FlyBase ID
FBrf0212018
Publication Type
Research paper
Abstract
The exon junction complex (EJC), which is deposited onto mRNAs as a consequence of splicing, is involved in multiple post-transcriptional events in metazoa. Here, using Drosophila melanogaster cells, we show that only some introns trigger EJC-dependent nonsense-mediated mRNA decay and that EJC association with particular spliced junctions depends on RNA cis-acting sequences. This study provides the first evidence to our knowledge that EJC deposition is not constitutive but instead is a regulated process.
