FB2026_02 , released June 18, 2026
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Pfeiffer, B., Rubin, G. (2014.4.16). Recombinase and tester constructs and insertions. 
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FBrf0224689
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The following information accompanied stocks donated to the Bloomington Stock Center by Barret Pfeiffer and Gerry Rubin, Janelia Farm Research Campus.
P{10XUAS(loxP.stop)GFP.Myr} and P{10XUAS(FRT.stop)GFP.Myr} are similar to the recombinase tester constructs described in Nern et al. (FBrf0214794).  A stop codon-bearing cassette is flanked by loxP or FRT sites so that a GFP variant (containing F64L and S65T amino acid substitutions and which has been optimized for Drosophila codon usage) with a myristoylation tag is expressed in the presence of Cre or FLP recombinase and an appropriate GAL4 driver.
P{3XUAS-RSR.PEST}is similar to the recombinase constructs described in Nern et al. R recombinase tagged with a PEST sequence is expressed under the control of three UAS copies.
P{UAS-B2R.PEST}, P{UAS-B3R.PEST}, P{UAS-KDR.PEST} and P{UAS-RSR.PEST} were constructed by cloning the PEST-tagged B2, B3, KD and R recombinase sequences described in Nern et al. into a vector similar to P{1XUAS-IVS- mCD8::GFP } (pJFRC3; FBtp0056314) in which the Hsp70 promoter had been replaced by a Drosophila synthetic core promoter (DSCP; FBrf0205679). P{UAS-cre.PEST} is a similar construct with PEST-tagged Cre.
P{10XUAS(loxP.stop)GFP.Myr}attP2, P{UAS-B2R.PEST}attP2, P{UAS-B3R.PEST}attP2, P{UAS-KDR.PEST}attP2, P{UAS-cre.PEST}attP2 and P{UAS-RSR.PEST}attP2 are insertions into P{CaryP}attP2 (FBti0040535) at 68A4,  3L:11063638  (R5).
P{10XUAS(loxP.stop)GFP.Myr}attP40 and P{3XUAS-RSR.PEST}attP40 are insertions into P{CaryP}attP40 (FBti0114379) at 25C6,  2L:5108448  (R5).
P{10XUAS(FRT.stop)GFP.Myr}attP18 is an insertion into P{CaryP}attP18 (FBti0114370) at 6C12,  X:6650355..6654052  (R5).
P{10XUAS(FRT.stop)GFP.Myr}su(Hw)attP5 is an insertion into P{CaryIP}su(Hw)attP5 (FBti0131990) at 50F1,  2R:10194873..10195195  (R5).
P{10XUAS(FRT.stop)GFP.Myr}su(Hw)attP1 is an insertion into P{CaryIP}su(Hw)attP1 (FBti0131259) at 87B13,  3R:8299797..8303038  (R5).
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