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Reference
Citation
Nern, A., Pfeiffer, B.D., Svoboda, K., Rubin, G.M. (2011). Multiple new site-specific recombinases for use in manipulating animal genomes.  Proc. Natl. Acad. Sci. U.S.A. 108(34): 14198--14203.
FlyBase ID
FBrf0214794
Publication Type
Research paper
Abstract

Site-specific recombinases have been used for two decades to manipulate the structure of animal genomes in highly predictable ways and have become major research tools. However, the small number of recombinases demonstrated to have distinct specificities, low toxicity, and sufficient activity to drive reactions to completion in animals has been a limitation. In this report we show that four recombinases derived from yeast--KD, B2, B3, and R--are highly active and nontoxic in Drosophila and that KD, B2, B3, and the widely used FLP recombinase have distinct target specificities. We also show that the KD and B3 recombinases are active in mice.

PubMed ID
PubMed Central ID
PMC3161616 (PMC) (EuropePMC)
Related Publication(s)
Personal communication to FlyBase

Constructs expressing FLP variants.
Pfeiffer and Rubin, 2014.4.17, Constructs expressing FLP variants. [FBrf0225079]

Commonly used Flippase Variants.
Pfeiffer, 2014.3.12, Commonly used Flippase Variants. [FBrf0225080]

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Secondary IDs
    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    Proc. Natl. Acad. Sci. U.S.A.
    Title
    Proceedings of the National Academy of Sciences of the United States of America
    Publication Year
    1915-
    ISBN/ISSN
    0027-8424
    Data From Reference
    Alleles (24)
    Genes (11)
    Natural transposons (1)
    Insertions (1)
    Experimental Tools (16)
    Transgenic Constructs (23)
    Transcripts (1)