Wong, K.K., Li, W., An, Y., Duan, Y., Li, Z., Kang, Y., Yan, Y. (2015). β-Spectrin Regulates the Hippo Signaling Pathway and Modulates the Basal Actin Network. J. Biol. Chem. 290(10): 6397--6407.
FlyBase ID
FBrf0227722
Publication Type
Research paper
Abstract
Emerging evidence suggests functional regulation of the Hippo pathway by the actin cytoskeleton, although the detailed molecular mechanism remains incomplete. In a genetic screen, we identified a requirement for β-Spectrin in the posterior follicle cells for the oocyte repolarization process during Drosophila mid-oogenesis. β-spectrin mutations lead to loss of Hippo signaling activity in the follicle cells. A similar reduction of Hippo signaling activity was observed after β-Spectrin knockdown in mammalian cells. We further demonstrated that β-spectrin mutations disrupt the basal actin network in follicle cells. The abnormal stress fiber-like actin structure on the basal side of follicle cells provides a likely link between the β-spectrin mutations and the loss of the Hippo signaling activity phenotype.
