The following information accompanied stocks donated to the Bloomington Stock Center by Tony Harris, University of Toronto.
P{UASp-GFP.CG15385} was constructed with pP{PGW} and expresses the CG15385 isoform A/B tagged at the N terminus with GFP.
P{UASp-GFP.CG45186} was constructed with pP{PGW} and expresses CG45186 protein tagged at the N terminus with GFP. It was originally constructed to correspond to a specific isoform of CG33232. CG33232 was reannotated as CG45186 and the sequence corresponds to amino acids 260 to 1111 of CG45186 isoform AA. The amino acid sequence is shown below:
MRSGIAERELRRLKLESYGHNANLAAEAIAGLASVEDFKSVALRSSSIPLQQMWLPHKPVMLLHVKGRTHVQTRLVEPVHTSLNRGDCFILVAGSQLFRYVGSFANVIEISRSKKICAAIVENKDLGCTATQEVILTDGKYLKERQWRQFWQLLGKKEDDQSDIAECGHADEDDVFESSLIETNKIYEYQDEALIPLEKFWGCIPKVDMLDTRKVLVFDFGSELYVWNGKNAPSDAKRAAMRLAQEHFGAENSADYADCYLNPLNYVAIVGRRENTKYAKRCAQRPEWCVLGKITQNMETVLFKEKFSDWPEIEREDLEKDYLSNGVHMVRALNGTALHKGEPYQEPNLVLEQANLGRGNFYYDTDTMRHFDVITKSTDKWQIHEFNFDAANNRDDYGHFYSAESYIVRWIYQISVTVRELSGKVSNRSTVGRDRCVYFTWQGQDSSANEKGAAALLTVELDKEKGAQMRVSQGDECTAFVRLFRQMWQHRGRKEQCLERHSEWRLYQLQGNVSEETIIKEVVCQSSHLRSRSSMLLIHGSQGNVIVWHGSKSAPHTRNVAVGVAQDLIKTVPKDLFSAETANLTEVEEGSDDDQCKKALGLKDERNEYGSLLKSTKSFDYQIRIFNFSSTQGVFKALELSDPLRCQDLHSPYPFSQSQLYNARQPTIFLLDDGDELWLWMGWWPLEDVKINTDERSSPTNENRAGVNRWISERRAALETAVDYWRAKHGENENEPFHGIKGQVVWAGLEPLAFKALFPDWTERPDVREINEEDGRTDASISIATMLAQMTQTEYPLEVLKARPLPEGVEPTRLEVYLNAEDFQAALGCSRAEFEQLPIWKQTKLKKERGLF
P{UASp-GFP.alph} was constructed with pP{PGW} and expresses the alph isoform E tagged at the N terminus with GFP.
P{UASp-GFP.RhoGAP19D} was constructed with pP{PGW} and expresses the RhoGAP19D isoform D tagged at the N terminus with GFP. The cDNA used to construct this transgene differs from the reference genome sequence by sequence changes encoding substitutions Q160H, S278T, T474S, T1509S and T2128S, deletion QQ265--, and insertions --371QQ, ---534QQ and -----572QQQQQ.
P{UASp-GFP.CG3880} was constructed with pP{PGW} and expresses CG3880 tagged at the N terminus with GFP.
P{UASp-mCherry.ena} was constructed with pP{PGW} with GFP replaced by mCherry and expresses ena isoform B tagged at the N terminus with mCherry. The cDNA used to construct this transgene differs from the reference sequence by sequence changes encoding substitutions Q120H and I652F and deletion QQQ349---.
P{UASp-mCherry.btsz} was constructed with pP{PGW} with GFP replaced by mCherry and expresses btsz isoform F tagged at the N terminus with mCherry. The cDNA used to construct this transgene differs from the reference sequence by sequence changes deleting M1 to R81.
P{UASp-hts.mCherry} was constructed with pP{PWG} with GFP replaced by mCherry and expresses hts isoform I tagged at the C terminus with mCherry. The cDNA used to construct this transgene differs from the reference sequence by sequence changes inserting Q at residue 76.
P{UASp-GFP.CG15385}attP2, P{UASp-GFP.CG45186}attP2, P{UASp-GFP.alph}attP2, P{UASp-GFP.RhoGAP19D}attP2, P{UASp-GFP.CG3880}attP2, P{UASp-mCherry.ena}attP2, P{UASp-mCherry.btsz}attP2 and P{UASp-hts.mCherry}attP2 are insertions in P{CaryP}attP2 at 68A4, 3L:11070538 (R6).