Kuznetsova, K.G., Kliuchnikova, A.A., Ilina, I.U., Chernobrovkin, A.L., Novikova, S.E., Farafonova, T.E., Karpov, D.S., Ivanov, M.V., Goncharov, A.O., Ilgisonis, E.V., Voronko, O.E., Nasaev, S.S., Zgoda, V.G., Zubarev, R.A., Gorshkov, M.V., Moshkovskii, S.A. (2018). Proteogenomics of Adenosine-to-Inosine RNA Editing in the Fruit Fly.  J. Proteome Res. 17(11): 3889--3903.

FBrf0240493

Research paper

Adenosine-to-inosine RNA editing is one of the most common types of RNA editing, a posttranscriptional modification made by special enzymes. We present a proteomic study on this phenomenon for Drosophila melanogaster. Three proteome data sets were used in the study: two taken from public repository and the third one obtained here. A customized protein sequence database was generated using results of genome-wide adenosine-to-inosine RNA studies and applied for identifying the edited proteins. The total number of 68 edited peptides belonging to 59 proteins was identified in all data sets. Eight of them being shared between the whole insect, head, and brain proteomes. Seven edited sites belonging to synaptic vesicle and membrane trafficking proteins were selected for validation by orthogonal analysis by Multiple Reaction Monitoring. Five editing events in cpx, Syx1A, Cadps, CG4587, and EndoA were validated in fruit fly brain tissue at the proteome level using isotopically labeled standards. Ratios of unedited-to-edited proteoforms varied from 35:1 ( Syx1A) to 1:2 ( EndoA). Lys-137 to Glu editing of endophilin A may have functional consequences for its interaction to membrane. The work demonstrates the feasibility to identify the RNA editing event at the proteome level using shotgun proteomics and customized edited protein database.