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Citation
Chen, Y.J., Chang, H.H., Lin, S.H., Lin, T.Y., Wu, T.H., Lin, H.J., Liou, N.F., Yang, C.J., Chen, Y.T., Chang, K.H., Li, C.Y., Chou, Y.H. (2019). Differential efficacy of genetically swapping GAL4.  J. Neurogenet. 33(2): 52--63.
FlyBase ID
FBrf0242708
Publication Type
Research paper
Abstract

Several large or mid-scale collections of Drosophila enhancer traps have been recently created to allow for genetic swapping of GAL4 coding sequences to versatile transcription activators or suppressors such as LexA, QF, split-GAL4 (GAL4-AD and GAL4-DBD), GAL80 and QS. Yet a systematic analysis of the feasibility and reproducibility of these tools is lacking. Here we focused on InSITE GAL4 drivers that specifically label different subpopulations of olfactory neurons, particularly local interneurons (LNs), and genetically swapped the GAL4 domain for LexA, GAL80 or QF at the same locus. We found that the major utility-limiting factor for these genetic swaps is that many do not fully reproduce the original GAL4 expression patterns. Different donors exhibit distinct efficacies for reproducing original GAL4 expression patterns. The successfully swapped lines reported here will serve as valuable reagents and expand the genetic toolkits of Drosophila olfactory circuit research.

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    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    J. Neurogenet.
    Title
    Journal of Neurogenetics
    Publication Year
    1983-
    ISBN/ISSN
    0167-7063
    Data From Reference
    Alleles (90)
    Genes (19)
    Insertions (43)
    Transgenic Constructs (50)