Abstract
Diesel exhaust particles (DEP), major air pollutants emitted from automobile engines, contain numerous toxic compounds. Although the adverse effects of DEP exposure on the respiratory and cardiovascular systems are well documented, its impact on brain health remains poorly understood. In this study, Drosophila melanogaster was used as a model organism to investigate the neurological effects of DEP exposure and the impact of exposure cessation across different age groups. Molecular, histopathological, and behavioral markers were assessed before and after exposure to varying DEP doses at different time intervals. DEP exposure induced age-dependent cellular responses in the brain, including elevated reactive oxygen species (ROS), increased neuroinflammation, and disruption of the blood-brain barrier (BBB). Prolonged exposure led to pronounced vacuolization in the brains of aged flies. Cessation of DEP exposure resulted in partial recovery in young flies, particularly when implemented early in life, whereas aged flies showed limited benefit, with persistent signs of likely irreversible brain damage. Overall, this findings support the need for greater public awareness and careful consideration in public health policies to limit long-term DEP exposure, especially among older individuals, and to promote strategies that reduce potential risks to brain health associated with air pollution.
