FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Insertion: Dmel\P{PZ}ken1
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General Information
Symbol
Dmel\P{PZ}ken1
Species
D. melanogaster
Name
FlyBase ID
FBti0003513
Feature type
transposable_element_insertion_site
Description
Inserted Element
P{PZ}
LINE ID

ms(2)00331

Affected gene(s)
Ecol\lacZ, ken
Viability / fertility
Associated allele(s)
Ecol\lacZken-1, ken1
Stock availability
1 publicly available
Genomic Location
Chromosomal Location
2R ( 60A6 )
Sequence Location
2R:23,870,309..23,870,320 [+]
Target / Docking site
Genomic Maps
Member of Large Scale Dataset(s)
Dataset
TI_set_P{PZ}.BDGP
Description

Insertion lines from this collection were assessed for inclusion in the Gene Disruption Project collection.

(Bellen et al., 2004)

A set of mutant stocks derived by insertional mutagenesis using the P-element construct P{PZ}; most lines have a lethal or sterile phenotype. The P{PZ} construct carries a ry[+] visible marker, Ecol\lacZ enhancer trap sequences, and bacterial sequences that allow plasmid rescue.

(Spradling et al., 1999)
Detailed Mapping Data
Chromosome (arm)
Sequence Location
2R:23,870,309..23,870,320 [+]
(Lukacsovich et al., 1999)
Orientation
Cytological location (computed by FlyBase)
60A6 (inferred by FlyBase from sequence location)
Cytological location (reported)
60A1-60A16 (in situ hybridization reported)
(BDGP Project Members, 1994-1999)
Insertion into Natural transposon
Comments concerning location
Sequence Data
Flanking sequence
AB010261
(FlyBase, 1992-)
Inserted Element
Construct
P{PZ}
Size (Kb)

14.545

Component Allele(s)
l(3)87Df7.2
ry+t7.2
Molecular map
Affected Gene(s)
Phenotypic Data
Expression Data
Reporter Expression
distribution deduced from reporter
Stage
Tissue/Position (including subcellular localization)
Reference
wandering third instar larval stage
embryonic/larval tracheal section
(Meister and Braun, 1995.10)
imaginal disc
(Meister and Braun, 1995.10)
larval brain
(Meister and Braun, 1995.10)
ring gland
(Meister and Braun, 1995.10)
embryonic/larval hindgut
(Meister and Braun, 1995.10)
adult stage | male
hub cell
(Issigonis and Matunis, 2012)
spermatogenesis & adult stage | male
male germline stem cell
(Issigonis and Matunis, 2012)
spermatogonium
(Issigonis and Matunis, 2012)
Additional Information
Statement
Reference

The ken1 enhancer trap allele is a slight hypomorph that drives beta-galactosidase expression in the hub, germline stem cells, and spermatogonia. ken expression was not readily detected by in-situ hybridization or immunofluorescence.

(Issigonis and Matunis, 2012)

In wandering third instar larva, high levels of β-gal expression in ring gland and imaginal discs (which discs not specified); moderate levels in the hindgut and trachea; low levels in the larval brain.

(Meister and Braun, 1995.10)
 
Marker for
Reflects expression of
ken
(Meister and Braun, 1995.10)
Reporter construct used in assay
Data on Genetic Line
Line ID

ms(2)00331

(Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
Progenitor(s) within the Genome
Related Aberration or Balancer
Aberration
Balancer
Stocks (1)
External Crossreferences and Linkouts ( 0 )
Comments
Comments

Site of host repeat sequence; The orientation of the insertion was not reported.

(Lukacsovich et al., 1999)

This insertion was listed in the BDGP database as a lethal or sterile line during the period 1994-1999, but was not verified as such prior to the summary publication (FBrf0111489). Reasons for excluding lines from the collection described in FBrf0111489 include presence of more than one P insertion on the mutant chromosome, separation of lethality (or sterility) from the location of the insertion, and loss of lethality (or sterility) from the stock. Further information is available from http://www.fruitfly.org/bfd/ and from Dr. Allan Spradling (spradling@mail1.ciwemb.edu).

(FlyBase, 1992-)
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
P{PZ}ken1
(Yoon et al., 2011, Lukacsovich et al., 1999, Castrillon et al., 1993, FlyBase, 1992-)
P{PZ}ms(2)00331
(FlyBase, 1992-)
P{ry+t7.2=PZ}ken1
(FlyBase, 1992-)
Name Synonyms
Secondary FlyBase IDs
  • FBti0000239
References (17)