FlyBase Allele Report: Dmel\ken[1]

(Ullastres et al., 2021, Yoon et al., 2011, Lukacsovich et al., 2003, Lukacsovich et al., 1999, Kuhnlein et al., 1998, Kolhekar et al., 1997, BDGP Project Members, 1994-1999, Castrillon et al., 1993)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

distribution deduced from reporter

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage | male

hub cell

(Issigonis and Matunis, 2012)

spermatogenesis & adult stage | male

male germline stem cell

(Issigonis and Matunis, 2012)

spermatogonium

(Issigonis and Matunis, 2012)

Additional Information

Statement

Reference

The ken¹ enhancer trap allele is a slight hypomorph that drives beta-galactosidase expression in the hub, germline stem cells, and spermatogonia. ken expression was not readily detected by in-situ hybridization or immunofluorescence.

(Issigonis and Matunis, 2012)

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal locomotor behavior | adult stage

(Allen et al., 2007)

abnormal locomotor behavior | adult stage (with Df(2R)or-BR11)

(Allen et al., 2007)

abnormal locomotor behavior | adult stage (with ken¹⁴¹)

(Allen et al., 2007)

abnormal neurophysiology | adult stage

(Allen et al., 2007)

abnormal neurophysiology | adult stage (with ken¹⁴¹)

(Allen et al., 2007)

abnormal phototaxis | adult stage

(Allen et al., 2007)

abnormal phototaxis | adult stage (with Df(2R)or-BR11)

(Allen et al., 2007)

abnormal phototaxis | adult stage (with ken¹⁴¹)

(Allen et al., 2007)

lethal - all die before end of pupal stage

(Arbouzova, 2006)

lethal - all die before end of pupal stage (with Df(2R)Chi^g230)

(Arbouzova, 2006)

partially lethal - majority die

some die during pupal stage

(Arbouzova, 2006)

some die during pupal stage (with Df(2R)Chi^g230)

(Arbouzova, 2006)

visible

(Lukacsovich et al., 2003)

visible | adult stage (with ken¹⁴¹)

(Allen et al., 2007)

Phenotype Manifest In

aedeagus

(Castrillon et al., 1993)

antenna

(Arbouzova, 2006)

arista

(Lukacsovich et al., 2003)

arista | adult stage (with ken¹⁴¹)

(Allen et al., 2007)

female analia

(Lukacsovich et al., 2003)

female genitalia

(Lukacsovich et al., 2003)

genitalia

(Arbouzova, 2006)

genitalia | adult stage (with ken¹⁴¹)

(Allen et al., 2007)

giant fiber neuron | adult stage

(Allen et al., 2007)

giant fiber neuron | adult stage (with ken¹⁴¹)

(Allen et al., 2007)

hypogynium

(Castrillon et al., 1993)

male genital disc (with ken^ok)

(Lukacsovich et al., 2003)

male genitalia

(Lukacsovich et al., 2003)

oviprovector

(Lukacsovich et al., 2003, Castrillon et al., 1993)

surstylus

(Castrillon et al., 1993)

terminalia (with ken⁰²⁹⁷⁰)

(Lukacsovich et al., 2003)

wing (with ken¹⁴¹)

(Allen et al., 2007)

Detailed Description

Statement

Reference

ken¹⁴¹/ken¹ transheterozygotes display the same adult phenotypes (abnormal, often missing, genitalia; unpigmented aristae; wings held out away from the body) characteristic or viable ken mutants. No obvious morphological defects are seen in the giant fiber neurons.

Homozygous ken¹ mutant adults as well as ken¹/Df(2R)or-BR11 hemizygotes and ken¹/ken¹⁴¹ transheterozygotes show defective light-off escape behavior, phototaxis and general locomotor activity compared to wild-type controls, with the hemizygotes showing the most severe phenotype in all assays.

Both adult ken¹ homozygotes and ken¹⁴¹/ken¹ transheterozygotes show defects in the electrophysiology of the giant fibre system: they display longer response latencies and a lower percentage following to 10 stimuli at 250Hz in tergo trochantal muscles compared to wild-type controls. When stimulated directly, the muscles of ken¹ mutants respond with the correct short latency characteristic for normal neuromuscular junctions.

(Allen et al., 2007)

Approximately 9.4% of the expected number of ken¹ homozygous mutants survive to adulthood and display antennal phenotypes and variable loss of genitalia structures.

Only 1% of the expected number of transheterozygous ken¹/Df(2R)Chi^g230 individuals eclose.

(Arbouzova, 2006)

A few mutant males and females have duplicated genitalia arranged side by side in a mirror image. Some mutant females lack a vulva and have a rotated anal plate. Homozygotes have unpigmented aristae that are somewhat frail physically. ken¹/ken⁰²⁹⁷⁰ escapers have abnormal terminalia (phenotype is 100% penetrant). The genital disc is malformed in ken¹/ken^ok mutant males and some discs are split into two subdivisions.

(Lukacsovich et al., 2003)

External genitalia are absent in some males and females. Aristae are sparse ands unpigmented. Semi-lethal, males and females are semi-sterile.

(Castrillon et al., 1993)

External genitalia absent from some males and females. Aristae sparse and unpigmented. male semi-sterile. female semi-sterile.

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Other

Statement

Reference

ken¹, upd1^GMR.PB has eye phenotype

(Arbouzova et al., 2006)

Additional Comments

Genetic Interactions

Statement

Reference

ken¹ heterozygous mutants enhance the upd1^GMR.PB eye overgrowth.

(Arbouzova et al., 2006)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

ken⁰²⁹⁷⁰

(Kuhnlein et al., 1998)

ken⁰⁸²⁵³

(Kuhnlein et al., 1998)

ken⁰²⁹⁷⁰

(BDGP Project Members, 1994-1999)

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

11753

cn¹ P{PZ}ken¹/CyO; ry⁵⁰⁶

Notes on Origin

Discoverer

A. Spradling.

Comments

Complements Phm^k07623.

(Kolhekar et al., 1997)

The strength of the following ken alleles can be classified from the weakest to the strongest as follows: ken¹ <= ken⁰²⁹⁷⁰ < ken^k11035 < Df(2R)Chi^g230.

(Arbouzova, 2006)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (6)

Reported As

Symbol Synonym

Ken¹

(Arbouzova, 2006)

P1753

(Kolhekar et al., 1997)

ken

(Lukacsovich et al., 1999)

ken⁰⁰³³¹

ken¹

(Gallois et al., 2024, Issigonis and Matunis, 2012, Christensen et al., 2008.9.3, Christensen et al., 2008.12.28, Allen et al., 2007, Arbouzova, 2006, Arbouzova et al., 2006)

ms(2)00331

(Beaton, 1999.12.12, Lukacsovich et al., 1999, Kuhnlein et al., 1998)

Name Synonyms

Secondary FlyBase IDs

FBal0043420

References (17)

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