FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Insertion: Dmel\P{lacW}k15501
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General Information
Symbol
Dmel\P{lacW}k15501
Species
D. melanogaster
Name
FlyBase ID
FBti0003931
Feature type
transposable_element_insertion_site
Description
Inserted Element
P{lacW}
LINE ID

l(2)k15501

Affected gene(s)
Amph, Ecol\lacZ, Sin3A, unch
Viability / fertility
Associated allele(s)
Ecol\lacZk15501
Stock availability
3 publicly available
Genomic Location
Chromosomal Location
2R ( 49B7 )
Sequence Location
2R:12,592,909..12,592,909 [+]
Target / Docking site
Genomic Maps
Member of Large Scale Dataset(s)
Dataset
TI_set_P{lacW}.BDGP
Description

Insertion lines from this collection were assessed for inclusion in the Gene Disruption Project collection.

(Bellen et al., 2004)

A set of mutant stocks derived by insertional mutagenesis using the P-element construct P{lacW}; most lines have a lethal or sterile phenotype. The P{lacW} construct carries a w+mC mini-white visible marker, Ecol\lacZ enhancer trap sequences, and bacterial sequences that allow plasmid rescue (FBrf0049800).

(Spradling et al., 1999)
Detailed Mapping Data
Chromosome (arm)
Sequence Location
2R:12,592,909..12,592,909 [+]
(BDGP Project Members, 1994-1999)
Orientation
Cytological location (computed by FlyBase)
49B7 (inferred by FlyBase from sequence location)
Cytological location (reported)
49B2--49B3 (in situ hybridization reported)
(Prokopenko et al., 2000)
49B3--49B6 (in situ hybridization reported)
(Bogart and Cook, 2000.11.8)
49B--49C (in situ hybridization reported)
(Kania et al., 1995)
49B1--49C4 (in situ hybridization reported)
(BDGP Project Members, 1994-1999)
Insertion into Natural transposon
Comments concerning location
Sequence Data
Inserted Element
Construct
P{lacW}
Size (Kb)

10.691

Component Allele(s)
w+mC
Molecular map
Affected Gene(s)
Phenotypic Data
Expression Data
Reporter Expression
distribution deduced from reporter
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage 16
embryonic/larval hindgut
(Kania et al., 1995)
ectoderm | segmentally repeated
(Kania et al., 1995)
embryonic/larval midgut
(Kania et al., 1995)
embryonic/larval pharynx
(Kania et al., 1995)
Additional Information
Statement
Reference

In embryo, expression in hindgut, midgut, pharynx and ectodermal stripes described as "basic" expression pattern; observed in many lines (FBrf0080145).

(Kania et al., 1995)
 
Marker for
Reflects expression of
Reporter construct used in assay
Data on Genetic Line
Line ID

l(2)k15501

(Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
Progenitor(s) within the Genome
Related Aberration or Balancer
Aberration
Balancer
Stocks (3)
External Crossreferences and Linkouts ( 0 )
Comments
Comments

Location 2R:8108816-8108817 confirmed by FlyBase alignment of dbGSS accession AQ026088 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. Insertion orientation confirmed.

(FlyBase, 2005)

Insertion is separable from the unchk15501 lethal mutation.

(Bogart and Cook, 2000.11.8)

Insertion maps ~24nt upstream of 5\' end of Sin3A mRNA.

(Prokopenko et al., 2000)

Insertion maps approximately 24bp upstream of the 5\' end of the longest Sin3A mRNA (GenBank accession: AF024603) and approximately 0.8kb upstream of the 5\' end of Amp.

(Prokopenko et al., 2000)
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
P{lacW}k15501
(FlyBase, 2005, Bogart and Cook, 2000.11.8, FlyBase, 1992-)
P{lacW}unchk15501
(Kania et al., 1995, FlyBase, 1992-)
Name Synonyms
Secondary FlyBase IDs
    References (11)