FlyBase Insertion Report: Dmel\P{PZ}retn[02535]

General Information

Symbol

Dmel\P{PZ}retn⁰²⁵³⁵

Species

D. melanogaster

Name

FlyBase ID

FBti0005219

Feature type

transposable_element_insertion_site

Description

Inserted Element

P{PZ}

LINE ID

l(2)02535

Affected gene(s)

Ecol\lacZ, retn

Viability / fertility

Associated allele(s)

Ecol\lacZ^retn-02535, retn⁰²⁵³⁵

Stock availability

1 publicly available

Genomic Location

Chromosomal Location

2R ( 59F5 )

Sequence Location

2R:23,632,588..23,632,588 [+]

Target / Docking site

Genomic Maps

JBrowse

Member of Large Scale Dataset(s)

Dataset

TI_set_P{PZ}.BDGP

Description

Insertion lines from this collection were assessed for inclusion in the Gene Disruption Project collection.

(Bellen et al., 2004)

A set of mutant stocks derived by insertional mutagenesis using the P-element construct P{PZ}; most lines have a lethal or sterile phenotype. The P{PZ} construct carries a ry[+] visible marker, Ecol\lacZ enhancer trap sequences, and bacterial sequences that allow plasmid rescue.

(Spradling et al., 1999)

Detailed Mapping Data

Chromosome (arm)

Sequence Location

2R:23,632,588..23,632,588 [+]

(BDGP Project Members, 1994-1999)

Orientation

Cytological location (computed by FlyBase)

59F5 (inferred by FlyBase from sequence location)

Cytological location (reported)

59F3-59F4 (in situ hybridization reported)

(BDGP Project Members, 1994-1999)

Insertion into Natural transposon

Comments concerning location

Sequence Data

Flanking sequence

AQ073271

(FlyBase, 1992-)

Inserted Element

Construct

P{PZ}

Size (Kb)

14.545

Component Allele(s)

l(3)87Df^7.2

ry^+t7.2

Molecular map

Affected Gene(s)

Insertion may affect gene

Ecol\lacZ

(Shandala et al., 1999, Harvie et al., 1998, Jackle and Janning, 1998.8.10, Vosshall, 1998.8.10, BDGP Project Members, 1994-1999)

retn

(BDGP Project Members, 1994-1999)

Variant Molecular Consequences

Phenotypic Data

Associated Allele

Phenotypic Data

Ecol\lacZ^retn-02535

Phenotypic Class Phenotype Manifest in Detailed Description

retn⁰²⁵³⁵

Phenotypic Class Phenotype Manifest in Detailed Description

Expression Data

Reporter Expression

distribution deduced from reporter

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage

wandering third instar larval stage

foregut imaginal ring

(Meister and Braun, 1995.10)

embryonic/larval hindgut

(Meister and Braun, 1995.10)

ring gland

(Harvie et al., 1998)

larval hindgut imaginal ring

(Meister and Braun, 1995.10)

Additional Information

Statement

Reference

In third instar larva, expression restricted to the ring gland.

(Harvie et al., 1998)

In embryo, expression in maxillary segment restricted to a posterior stripe; expression in PNS and salivary gland duct not certain; expression in proventriculus may correspond to hindgut imaginal ring.

(Jackle and Janning, 1998.8.10)

Marker for

ring gland

(Harvie et al., 1998)

Reflects expression of

Reporter construct used in assay

Data on Genetic Line

Line ID

l(2)02535

(Gene Disruption Project members, 2001-)

Origin as a multiple insertion line

Progenitor(s) within the Genome

Related Aberration or Balancer

Aberration

Balancer

Stocks (1)

Bloomington

11200

cn¹ P{PZ}retn⁰²⁵³⁵/CyO; ry⁵⁰⁶

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

P1200

(Meister and Braun, 1995.10)

P{PZ}dri⁰²⁵³⁵

(FlyBase, 1992-)

P{PZ}l(2)02535⁰²⁵³⁵

(BDGP Project Members, 1994-1999, FlyBase, 1992-)

P{PZ}retn⁰²⁵³⁵

(FlyBase, 1992-)

P{ry^+t7.2=PZ}l(2)02535⁰²⁵³⁵

(FlyBase, 1992-)

Name Synonyms

Secondary FlyBase IDs

FBti0000298

References (12)

Report Sections

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