FlyBase Insertion Report: Dmel\P{PZ}Rab6[08323]

General Information

Symbol

Dmel\P{PZ}Rab6⁰⁸³²³

Species

D. melanogaster

Name

FlyBase ID

FBti0005337

Feature type

transposable_element_insertion_site

Description

Inserted Element

P{PZ}

LINE ID

l(2)08323

Affected gene(s)

Ecol\lacZ, Rab6

Viability / fertility

Associated allele(s)

Ecol\lacZ^Rab6-08323, Rab6⁰⁸³²³

Stock availability

1 publicly available

Genomic Location

Chromosomal Location

2L ( 33C4 )

Sequence Location

2L:12,108,824..12,108,833 [-]

Target / Docking site

Genomic Maps

JBrowse

Member of Large Scale Dataset(s)

Dataset

TI_set_P{PZ}.BDGP

Description

Insertion lines from this collection were assessed for inclusion in the Gene Disruption Project collection.

(Bellen et al., 2004)

A set of mutant stocks derived by insertional mutagenesis using the P-element construct P{PZ}; most lines have a lethal or sterile phenotype. The P{PZ} construct carries a ry[+] visible marker, Ecol\lacZ enhancer trap sequences, and bacterial sequences that allow plasmid rescue.

(Spradling et al., 1999)

Detailed Mapping Data

Chromosome (arm)

Sequence Location

2L:12,108,824..12,108,833 [-]

(Purcell and Artavanis-Tsakonas, 1999)

Orientation

Cytological location (computed by FlyBase)

33C4 (inferred by FlyBase from sequence location)

Cytological location (reported)

33D1-33D2 (in situ hybridization reported)

(BDGP Project Members, 1994-1999)

Insertion into Natural transposon

Comments concerning location

Genomic location previously reported as 2L:12108826..12108826 .

Sequence Data

Flanking sequence

G00615

(FlyBase, 1992-)

Inserted Element

Construct

P{PZ}

Size (Kb)

14.545

Component Allele(s)

l(3)87Df^7.2

ry^+t7.2

Molecular map

Affected Gene(s)

Insertion may affect gene

Ecol\lacZ

(Vosshall, 1998.8.10, Meister and Braun, 1995.10, BDGP Project Members, 1994-1999)

Rab6

(BDGP Project Members, 1994-1999)

Variant Molecular Consequences

Phenotypic Data

Associated Allele

Phenotypic Data

Ecol\lacZ^Rab6-08323

Phenotypic Class Phenotype Manifest in Detailed Description

Rab6⁰⁸³²³

Phenotypic Class Phenotype Manifest in Detailed Description

Expression Data

Reporter Expression

distribution deduced from reporter

Stage

Tissue/Position (including subcellular localization)

Reference

wandering third instar larval stage

gonad

(Meister and Braun, 1995.10)

embryonic/larval foregut

(Meister and Braun, 1995.10)

embryonic/larval fat body

(Meister and Braun, 1995.10)

embryonic/larval midgut

(Meister and Braun, 1995.10)

Malpighian tubule

(Meister and Braun, 1995.10)

embryonic/larval hindgut

(Meister and Braun, 1995.10)

larval brain

(Meister and Braun, 1995.10)

imaginal disc

(Meister and Braun, 1995.10)

embryonic/larval salivary gland

(Meister and Braun, 1995.10)

adult stage

third segment of antenna

(Vosshall, 1998.8.10)

Additional Information

Statement

Reference

Weak β-gal expression in many tissue in wandering third instar larva; stronger expression in the imaginal discs (which discs not specified) and the Malpighian tubules.

(Meister and Braun, 1995.10)

Marker for

Reflects expression of

Reporter construct used in assay

Data on Genetic Line

Line ID

l(2)08323

(Gene Disruption Project members, 2001-)

Origin as a multiple insertion line

Progenitor(s) within the Genome

Related Aberration or Balancer

Aberration

Balancer

Stocks (1)

Bloomington

12352

cn¹ P{PZ}Rab6⁰⁸³²³/CyO; ry⁵⁰⁶

External Crossreferences and Linkouts ( 0 )

Comments

stated to be inserted 30 bp upstream from the start methionine; the range from 25 to 35 base pair upstream from the start methionine is shown

(Purcell and Artavanis-Tsakonas, 1999)

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

P{PZ}Rab6⁰⁸³²³

(Purcell and Artavanis-Tsakonas, 1999, FlyBase, 1992-)

P{PZ}l(2)08323⁰⁸³²³

(BDGP Project Members, 1994-1999, FlyBase, 1992-)

P{ry^+t7.2=PZ}l(2)08323⁰⁸³²³

(FlyBase, 1992-)

Name Synonyms

Secondary FlyBase IDs

FBti0000422

References (10)

Report Sections

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