FlyBase Insertion Report: Dmel\P{PZ}CycE[05206]

General Information

Symbol

Dmel\P{PZ}CycE⁰⁵²⁰⁶

Species

D. melanogaster

Name

FlyBase ID

FBti0005357

Feature type

transposable_element_insertion_site

Description

Inserted Element

P{PZ}

LINE ID

l(2)05206

Affected gene(s)

CycE, Ecol\lacZ

Viability / fertility

viable

Associated allele(s)

CycE⁰⁵²⁰⁶, Ecol\lacZ^CycE-05206

Stock availability

1 publicly available

Genomic Location

Chromosomal Location

2L ( 35D4 )

Sequence Location

2L:15,745,542..15,745,587 [+]

Target / Docking site

Genomic Maps

JBrowse

Member of Large Scale Dataset(s)

Dataset

TI_set_P{PZ}.BDGP

Description

Insertion lines from this collection were assessed for inclusion in the Gene Disruption Project collection.

(Bellen et al., 2004)

A set of mutant stocks derived by insertional mutagenesis using the P-element construct P{PZ}; most lines have a lethal or sterile phenotype. The P{PZ} construct carries a ry[+] visible marker, Ecol\lacZ enhancer trap sequences, and bacterial sequences that allow plasmid rescue.

(Spradling et al., 1999)

Detailed Mapping Data

Chromosome (arm)

Sequence Location

2L:15,745,542..15,745,587 [+]

(Sekelsky et al., 1999, Knoblich et al., 1994)

Orientation

Cytological location (computed by FlyBase)

35D4 (inferred by FlyBase from sequence location)

Cytological location (reported)

35D3-35D4 (in situ hybridization reported)

(BDGP Project Members, 1994-1999)

Insertion into Natural transposon

Comments concerning location

Sequence Data

Flanking sequence

G00412

(FlyBase, 1992-)

Inserted Element

Construct

P{PZ}

Size (Kb)

14.545

Component Allele(s)

l(3)87Df^7.2

ry^+t7.2

Molecular map

Affected Gene(s)

Insertion may affect gene

CycE

(Jagut et al., 2013, Geng and MacDonald, 2007, Knoblich et al., 1994)

Ecol\lacZ

(Jackle and Janning, 1998.8.10, Vosshall, 1998.8.10, Meister and Braun, 1995.10, BDGP Project Members, 1994-1999)

Variant Molecular Consequences

Phenotypic Data

Associated Allele

Phenotypic Data

CycE⁰⁵²⁰⁶

Phenotypic Class Phenotype Manifest in Detailed Description

Ecol\lacZ^CycE-05206

Phenotypic Class Phenotype Manifest in Detailed Description

Expression Data

Reporter Expression

distribution deduced from reporter

Stage

Tissue/Position (including subcellular localization)

Reference

wandering third instar larval stage

larval brain

(Meister and Braun, 1995.10)

imaginal disc

(Meister and Braun, 1995.10)

Additional Information

Statement

Reference

In wandering third instar, relatively weak expression in larval brain; strong expression in imaginal discs. (Not specified which imaginal discs.)

(Meister and Braun, 1995.10)

Marker for

Reflects expression of

CycE

(Meister and Braun, 1995.10)

Reporter construct used in assay

Data on Genetic Line

Line ID

l(2)05206

(Gene Disruption Project members, 2001-)

Origin as a multiple insertion line

Progenitor(s) within the Genome

Related Aberration or Balancer

Aberration

Balancer

Stocks (1)

Bloomington

11396

P{PZ}CycE⁰⁵²⁰⁶ cn¹/CyO; ry⁵⁰⁶

External Crossreferences and Linkouts ( 0 )

Comments

The P{PZ}CycE⁰⁵²⁰⁶ insertion site is within 20bp of the target site of P{lacW}CycE^P2.

(Sekelsky et al., 1999, Knoblich et al., 1994)

Synonyms and Secondary IDs (7)

Reported As

Symbol Synonym

05206

(Geng and MacDonald, 2007)

P{PZ}05206

(Ashburner et al., 1999)

P{PZ}CycE⁰⁵²⁰⁶

(Sekelsky et al., 1999, Knoblich et al., 1994, FlyBase, 1992-)

P{PZ}l(2)05206

(FlyBase, 1992-)

P{ry^+t7.2=PZ}CycE⁰⁵²⁰⁶

(FlyBase, 1992-)

cycE-lacZ

(Wang et al., 2018)

cycE⁰⁵²⁰⁶-lacZ

(Wang et al., 2018)

Name Synonyms

Secondary FlyBase IDs

FBti0000193

References (32)

Report Sections

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