FlyBase Insertion Report: Dmel\P{PZ}eIF4E1[07238]

General Information

Symbol

Dmel\P{PZ}eIF4E1⁰⁷²³⁸

Species

D. melanogaster

Name

FlyBase ID

FBti0005584

Feature type

transposable_element_insertion_site

Description

Inserted Element

P{PZ}

LINE ID

l(3)07238

Affected gene(s)

Ecol\lacZ, eIF4E1

Viability / fertility

Associated allele(s)

Ecol\lacZ^eIF4E1-07238, eIF4E1⁰⁷²³⁸

Stock availability

1 publicly available

Genomic Location

Chromosomal Location

3L ( 67B4 )

Sequence Location

3L:9,401,969..9,401,969 [+]

Target / Docking site

Genomic Maps

JBrowse

Member of Large Scale Dataset(s)

Dataset

TI_set_P{PZ}.BDGP

Description

Insertion lines from this collection were assessed for inclusion in the Gene Disruption Project collection.

(Bellen et al., 2004)

A set of mutant stocks derived by insertional mutagenesis using the P-element construct P{PZ}; most lines have a lethal or sterile phenotype. The P{PZ} construct carries a ry[+] visible marker, Ecol\lacZ enhancer trap sequences, and bacterial sequences that allow plasmid rescue.

(Spradling et al., 1999)

Detailed Mapping Data

Chromosome (arm)

Sequence Location

3L:9,401,969..9,401,969 [+]

(BDGP Project Members, 1994-1999)

Orientation

Cytological location (computed by FlyBase)

67B4 (inferred by FlyBase from sequence location)

Cytological location (reported)

67B1-67B2 (in situ hybridization reported)

(BDGP Project Members, 1994-1999)

Insertion into Natural transposon

Comments concerning location

Sequence Data

Flanking sequence

AQ034073

(FlyBase, 1992-)

Inserted Element

Construct

P{PZ}

Size (Kb)

14.545

Component Allele(s)

l(3)87Df^7.2

ry^+t7.2

Molecular map

Affected Gene(s)

Insertion may affect gene

Ecol\lacZ

(Harvie et al., 1998, Vosshall, 1998.8.10, BDGP Project Members, 1994-1999)

eIF4E1

(Sofola et al., 2007, BDGP Project Members, 1994-1999)

Variant Molecular Consequences

Phenotypic Data

Associated Allele

Phenotypic Data

Ecol\lacZ^eIF4E1-07238

Phenotypic Class Phenotype Manifest in Detailed Description

eIF4E1⁰⁷²³⁸

Phenotypic Class Phenotype Manifest in Detailed Description

Expression Data

Reporter Expression

distribution deduced from reporter

Stage

Tissue/Position (including subcellular localization)

Reference

wandering third instar larval stage

imaginal disc

(Harvie et al., 1998, Meister and Braun, 1995.10)

embryonic/larval optic lobe

(Harvie et al., 1998)

larval ventral nerve cord

(Harvie et al., 1998)

ring gland

(Harvie et al., 1998)

adult stage

adult brain | restricted

(Vosshall, 1998.8.10)

adult central complex

(Vosshall, 1998.8.10)

Additional Information

Statement

Reference

In the adult, low level of expression in the central brain.

(Vosshall, 1998.8.10)

In wandering third instar larva, expression in imaginal discs (which discs not specified).

(Meister and Braun, 1995.10)

Marker for

Reflects expression of

Reporter construct used in assay

Data on Genetic Line

Line ID

l(3)07238

(Gene Disruption Project members, 2001-)

Origin as a multiple insertion line

Progenitor(s) within the Genome

Related Aberration or Balancer

Aberration

Balancer

Stocks (1)

Bloomington

11720

P{PZ}eIF4E1⁰⁷²³⁸ ry⁵⁰⁶/TM3, ry^RK Sb¹ Ser¹

External Crossreferences and Linkouts ( 0 )

Comments

Location 3L:9375916-9375917 confirmed by FlyBase alignment of dbGSS accession AQ034073 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. Insertion orientation confirmed.

(FlyBase, 2005)

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

P1720

(Meister and Braun, 1995.10)

P{PZ}eIF-4E⁰⁷²³⁸

(FlyBase, 2005, FlyBase, 1992-)

P{PZ}eIF4E1⁰⁷²³⁸

P{PZ}l(3)07238⁰⁷²³⁸

(BDGP Project Members, 1994-1999, FlyBase, 1992-)

P{ry^+t7.2=PZ}l(3)07238⁰⁷²³⁸

(FlyBase, 1992-)

Name Synonyms

Secondary FlyBase IDs

FBti0000638

References (15)

Report Sections

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