FlyBase Insertion Report: Dmel\P{PZ}Gprk2[06936]

General Information

Symbol

Dmel\P{PZ}Gprk2⁰⁶⁹³⁶

Species

D. melanogaster

Name

FlyBase ID

FBti0006267

Feature type

transposable_element_insertion_site

Description

Inserted Element

P{PZ}

LINE ID

l(3)06936

fs(3)06936

Affected gene(s)

Ecol\lacZ, Gprk2, PNPase

Viability / fertility

Associated allele(s)

Ecol\lacZ^Gprk2-06936, Gprk2⁰⁶⁹³⁶, PNPase⁰⁶⁹³⁶

Stock availability

1 publicly available

Genomic Location

Chromosomal Location

3R ( 100C3 )

Sequence Location

3R:31,405,416..31,405,416 [+]

Target / Docking site

Genomic Maps

JBrowse

Member of Large Scale Dataset(s)

Dataset

TI_set_P{PZ}.BDGP

Description

Insertion lines from this collection were assessed for inclusion in the Gene Disruption Project collection.

(Bellen et al., 2004)

A set of mutant stocks derived by insertional mutagenesis using the P-element construct P{PZ}; most lines have a lethal or sterile phenotype. The P{PZ} construct carries a ry[+] visible marker, Ecol\lacZ enhancer trap sequences, and bacterial sequences that allow plasmid rescue.

(Spradling et al., 1999)

Detailed Mapping Data

Chromosome (arm)

Sequence Location

3R:31,405,416..31,405,416 [+]

(BDGP Project Members, 1994-1999)

Orientation

Cytological location (computed by FlyBase)

100C3 (inferred by FlyBase from sequence location)

Cytological location (reported)

100C-89E (in situ hybridization reported)

(BDGP Project Members, 1994-1999)

Insertion into Natural transposon

Comments concerning location

Genomic location previously reported as 3R:27231129..27231137 .

Sequence Data

Flanking sequence

AQ025576

(FlyBase, 1992-)

Inserted Element

Construct

P{PZ}

Size (Kb)

14.545

Component Allele(s)

l(3)87Df^7.2

ry^+t7.2

Molecular map

Affected Gene(s)

Insertion may affect gene

Ecol\lacZ

(Cheng et al., 2010, Molnar et al., 2007, Mohit et al., 2006, BDGP Project Members, 1994-1999)

Gprk2

(Schneider and Spradling, 1997)

PNPase

(FlyBase, 1996-)

Variant Molecular Consequences

Phenotypic Data

Associated Allele

Phenotypic Data

Ecol\lacZ^Gprk2-06936

Phenotypic Class Phenotype Manifest in Detailed Description

Gprk2⁰⁶⁹³⁶

Phenotypic Class Phenotype Manifest in Detailed Description

PNPase⁰⁶⁹³⁶

Phenotypic Class Phenotype Manifest in Detailed Description

Expression Data

Reporter Expression

distribution deduced from reporter

Stage

Tissue/Position (including subcellular localization)

Reference

third instar larval stage

anterior-posterior compartment boundary of the wing disc

(Cheng et al., 2010)

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Data on Genetic Line

Line ID

l(3)06936

(Gene Disruption Project members, 2001-)

fs(3)06936

(Gene Disruption Project members, 2001-)

Origin as a multiple insertion line

Progenitor(s) within the Genome

Related Aberration or Balancer

Aberration

Balancer

Stocks (1)

Bloomington

44625

w^*; P{neoFRT}82B Df(3R)Su(fu)^LP, Su(fu)^LP, P{PZ}Gprk2⁰⁶⁹³⁶ PNPase⁰⁶⁹³⁶/TM6B, Tb¹

External Crossreferences and Linkouts ( 0 )

Comments

Location 3R:27231138-27231139 confirmed by FlyBase alignment of dbGSS accession AQ025576 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b.

(FlyBase, 2005)

P element insertion is in reverse orientation with respect to Gprk2 gene transcription.

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

PZ-Gprk2⁰⁶⁹³⁶

(Molnar et al., 2007)

P{PZ}Gprk2⁰⁶⁹³⁶

(Mohit et al., 2006, FlyBase, 2005, Schneider and Spradling, 1997, FlyBase, 1992-)

P{PZ}Gprk2⁶⁹³⁶

(Schneider and Spradling, 1997, FlyBase, 1992-)

gprk2-LacZ

(Cheng et al., 2010)

l(3)06936

(FlyBase, 1992-)

Name Synonyms

Secondary FlyBase IDs

FBti0004448

References (18)

Report Sections

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