FB2026_02 , released June 18, 2026
Allele: Dmel\Gprk206936
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General Information
Symbol
Dmel\Gprk206936
Species
D. melanogaster
Name
FlyBase ID
FBal0063239
Feature type
allele
Associated gene
Dmel\Gprk2
Associated Insertion(s)
P{PZ}Gprk206936
Carried in Construct
Also Known As
gprk26936, fs(3)06936, P{PZ}Gprk206936
Key Links
Genomic Maps

Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
P-element activity
(Spradling et al., 1999, Schneider and Spradling, 1997, FlyBase, 1996-, BDGP Project Members, 1994-1999)
Progenitor genotype
Associated Insertion(s)
P{PZ}Gprk206936
Cytology
Description

Insertion of a P{PZ} element in the 5' untranslated region. The P{PZ} element is oriented in the same orientation as the direction of transcription of Gprk2.

(Schneider and Spradling, 1997)

The P{PZ}Gprk206936 insertion maps within both Gprk2 and CG11337 (overlapping genes on opposite strands).

(FlyBase, 1996-)
Allele components
Component
Use(s)
Inserted element
P{PZ}
Encoded product / tool
lacZ
Tagged with
Tag:NLS(P)
Mutations Mapped to the Genome
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Embryos maternally and zygotically mutant for Gprk206936 display a rounded overall shape. Cellularization proceeds normally but abnormal gastrulation is observed. The apical constricting area is expanded to the entire mesoderm and the width of the mesodermal region reduces more quickly than in wild type. However, this involution ceases prematurely and never resumes. Myosin accumulation starts earlier in Gprk206936 mutant mesodermal cells compared to controls and continues to accumulate on the apical surface. Ectodermal cells on the sides of the mesoderm extend apical protrusions but these never undergo involution movement. At later stages (germ band extension) the ventral furrow frequently remains open as a U-shaped furrow in the mutant, and about 2/3 of the mutant embryos have mesoderm exposed to the outside. Germ cells are also abnormally positioned at this stage.

      (Fuse et al., 2013)

      Gprk206936 flies have a constant low-amplitude electroantennogram (EAG) response to ethyl acetate that is near the wild-type peak value, in contrast to wild-type flies, which show robust EAG responses during the middle of the night but weak responses during the middle of the day.

      (Tanoue et al., 2008)

      Crosses of heterozygous flies produce only 21-34% of the expected number of progeny. The rate of egg laying is reduced in homozygous females; they lay 58% as many eggs as wild-type females. The hatching rate of eggs derived from homozygous females mated to wild-type males is about 46%. The hatching rate of eggs derived from homozygous females mated to homozygous males is 4%. The hatching rate of eggs derived from wild-type females mated to homozygous males is 84%. Homozygous females and females containing homozygous germline clones produce eggs with malformed dorsal appendages and with nurse cells that have not completed cytoplasmic dumping.

      (Fan and Schneider, 2003)

      Nurse cell dumping is incomplete in egg chambers from homozygous females and dorsal appendages are malformed. Some nuclei of nurse cells have a defect in tethering during cytoplasmic dumping; in 31% of stage 10B homozygous egg chambers nuclei are seen stretching in the direction of the cytoplasmic dumping and extending through the ring canals (this is not seen in wild type). No more than two nuclei are affected in a single egg chamber, and the nurse cells that dump directly into the oocyte are usually normal. In wild-type stage 10B egg chambers, actin forms a cage around each nucleus, in homozygous egg chambers the actin filaments are still present but are disorganised, with clumping of actin in some nurse cells. Homozygous females mated to wild-type males lay 1.2 eggs/fly/hour compared to 2.9 eggs/fly/hour for wild-type females. Only 21.3% of these eggs hatch.

      (Lannutti and Schneider, 2001)

      Homozygous egg chambers degenerate during vitellogenic stages (stages 8 to 10A) at a higher frequency than expected; 26.8% of ovarioles from 4 day old homozygous females contain degenerating egg chambers, compared to 0.7% in wild-type females. 5.2% of homozygous ovarioles contain fewer egg chambers than normal. These ovarioles often contain smaller, thinner germaria than normal. Mature oocytes produced by homozygous females are slightly shorter and more rounded than wild-type. The dorsal appendages are positioned normally, although they are shorter and broader than wild-type and are often different lengths. The operculum is oriented more vertically than normal. The micropyle is present. Nurse cells often fail to completely transfer their contents to the oocyte. Homozygous females lay a small number of eggs, 10.3% of which hatch following crosses to homozygous males, and 23.7% of which hatch following crosses to wild-type males. The unhatched eggs show a number of defects including twisted gastrulation, fused adjacent segments, and perforated dorsal and ventral cuticle. These defects are more severe in embryos lacking both maternal and zygotic Gprk2+ function.

      (Schneider and Spradling, 1997)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Suppressed by
      Statement
      Reference

      Gprk206936 has abnormal cell migration | embryonic stage phenotype, suppressible by fog4

      (Fuse et al., 2013)

      Gprk206936 has abnormal cell migration | embryonic stage phenotype, suppressible by fog4/fog[+]

      (Fuse et al., 2013)

      Gprk206936 has female sterile | recessive phenotype, suppressible by Pde4dnc-M14/dnc[+]

      (Lannutti and Schneider, 2001)

      Gprk206936 has female sterile | recessive phenotype, suppressible by Pde4dnc-2/dnc[+]

      (Lannutti and Schneider, 2001)
      Enhancer of
      Statement
      Reference

      Gprk2[+]/Gprk206936 is an enhancer of visible | adult stage phenotype of Scer\GAL4C-765, smoPKA12.UAS

      (Chen et al., 2010)
      Suppressor of
      NOT Suppressor of
      Statement
      Reference

      Gprk2[+]/Gprk206936 is a non-suppressor of partially lethal - majority die | recessive phenotype of Pde4dnc-2

      (Lannutti and Schneider, 2001)
      Phenotype Manifest In
      Suppressed by
      Statement
      Reference

      Gprk206936 has ventral furrow phenotype, suppressible by fog4

      (Fuse et al., 2013)

      Gprk206936 has embryo | embryonic stage 6 phenotype, suppressible by fog4/fog[+]

      (Fuse et al., 2013)

      Gprk206936 has ventral furrow phenotype, suppressible by fog4/fog[+]

      (Fuse et al., 2013)

      Gprk206936 has embryo | embryonic stage 6 phenotype, suppressible by fog4

      (Fuse et al., 2013)

      Gprk206936 has nurse cell & nucleus phenotype, suppressible by Pde4dnc-2/dnc[+]

      (Lannutti and Schneider, 2001)

      Gprk206936 has nurse cell & nucleus phenotype, suppressible by Pde4dnc-M14/dnc[+]

      (Lannutti and Schneider, 2001)
      Enhancer of
      Statement
      Reference

      Gprk2[+]/Gprk206936 is an enhancer of wing blade phenotype of Scer\GAL4C-765, smoPKA12.UAS

      (Chen et al., 2010)

      Gprk2[+]/Gprk206936 is an enhancer of wing vein phenotype of Scer\GAL4C-765, smoPKA12.UAS

      (Chen et al., 2010)
      Suppressor of
      Statement
      Reference

      Gprk2[+]/Gprk206936 is a suppressor of egg chamber phenotype of Pde4dnc-2

      (Lannutti and Schneider, 2001)

      Gprk2[+]/Gprk206936 is a suppressor of egg chamber phenotype of Pde4dnc-M14

      (Lannutti and Schneider, 2001)

      Gprk2[+]/Gprk206936 is a suppressor | partially of egg chamber phenotype of Pde4dnc-2/Pde4dnc-M14

      (Lannutti and Schneider, 2001)
      NOT Suppressor of
      Statement
      Reference

      Gprk206936 is a non-suppressor of ventral furrow phenotype of fog4

      (Fuse et al., 2013)
      Other
      Statement
      Reference

      Gprk206936, fog4 has ventral furrow phenotype

      (Fuse et al., 2013)
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Homozygous fog4 suppresses the gastrulation phenotype seen in Gprk206936 mutant embryos. As in fog4 mutant embryos alone, only a few apically constricting cells are seen at random positions. When only one copy of Gprk206936 is present apical constriction is substantially disorganised and asynchronous among mesodermal cells.

      (Fuse et al., 2013)

      The 'fused wing' phenotype (partial fusion between L3 and L4 veins) observed in flies expressing smoPKA12.Scer\UAS under the control of Scer\GAL4C-765 is enhanced by combination with Gprk206936 in heterozygous state, which results in severe vein defects.

      (Chen et al., 2010)

      The egg laying and hatching defects of Gprk206936 homozygous females are partially suppressed by a single copy of dnc2 or dncM14. The defect in tethering of the nurse cell nuclei seen in Gprk206936 homozygous egg chambers is partially suppressed by a single copy of dnc2 or dncM14. dnc2/dnc2 females carrying Gprk206936/+ lay 1.3 eggs/female/hour (wild-type value is 2.9 eggs/female/hour) of which 32.3% hatch. dnc2/dncM14 females carrying Gprk206936/+ lay 1.5 eggs/female/hour of which 31.6% hatch. dncM14/dncM14 females carrying Gprk206936/+ lay 1.0 eggs/female/hour (wild-type value is 2.9 eggs/female/hour) of which 38.3% hatch. The egg chamber degeneration of dnc2 and dncM14 homozygotes and dnc2/dncM14 females is partially suppressed by Gprk206936/+.

      (Lannutti and Schneider, 2001)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (5)
      References (13)