FlyBase Insertion Report: Dmel\P{lacW}Fatp1[k10307]

General Information

Symbol

Dmel\P{lacW}Fatp1^k10307

Species

D. melanogaster

Name

FlyBase ID

FBti0006518

Feature type

transposable_element_insertion_site

Description

Inserted Element

P{lacW}

LINE ID

l(2)k10307

Affected gene(s)

Ecol\lacZ, Fatp1

Viability / fertility

Associated allele(s)

Ecol\lacZ^Fatp1-k10307, Fatp1^k10307

Stock availability

2 publicly available

Genomic Location

Chromosomal Location

2L ( 31F5 )

Sequence Location

2L:10,516,565..10,516,565 [+]

Target / Docking site

Genomic Maps

JBrowse

Member of Large Scale Dataset(s)

Dataset

TI_set_P{lacW}.BDGP

Description

Insertion lines from this collection were assessed for inclusion in the Gene Disruption Project collection.

(Bellen et al., 2004)

A set of mutant stocks derived by insertional mutagenesis using the P-element construct P{lacW}; most lines have a lethal or sterile phenotype. The P{lacW} construct carries a w^+mC mini-white visible marker, Ecol\lacZ enhancer trap sequences, and bacterial sequences that allow plasmid rescue (FBrf0049800).

(Spradling et al., 1999)

Detailed Mapping Data

Chromosome (arm)

Sequence Location

2L:10,516,565..10,516,565 [+]

(BDGP Project Members, 1994-1999)

Orientation

Cytological location (computed by FlyBase)

31F5 (inferred by FlyBase from sequence location)

Cytological location (reported)

31F4-31F5 (in situ hybridization reported)

(BDGP Project Members, 1994-1999)

Insertion into Natural transposon

Comments concerning location

Sequence Data

Flanking sequence

AQ034026

(FlyBase, 1992-)

Inserted Element

Construct

P{lacW}

Size (Kb)

10.691

Component Allele(s)

w^+mC

Molecular map

Affected Gene(s)

Insertion may affect gene

Ecol\lacZ

(BDGP Project Members, 1994-1999)

Fatp1

(BDGP Project Members, 1994-1999)

Variant Molecular Consequences

Phenotypic Data

Associated Allele

Phenotypic Data

Ecol\lacZ^Fatp1-k10307

Phenotypic Class Phenotype Manifest in Detailed Description

Fatp1^k10307

Phenotypic Class Phenotype Manifest in Detailed Description

Expression Data

Reporter Expression

distribution deduced from reporter

Stage

Tissue/Position (including subcellular localization)

Reference

third instar larval stage

embryonic/larval midgut

(Dourlen et al., 2012)

embryonic/larval proventriculus | posterior | restricted

(Dourlen et al., 2012)

adult stage

eye photoreceptor cell

(Dourlen et al., 2012)

secondary pigment cell

(Dourlen et al., 2012)

tertiary pigment cell

(Dourlen et al., 2012)

adult optic lobe | restricted

(Dourlen et al., 2012)

Additional Information

Statement

Reference

Fatp1^k10307 drives expression in the secondary and tertiary pigment cells, and in neurons in the adult retina. Expression is also detected at the level of the photoreceptors and in cells around the medulla and lamina.

(Dourlen et al., 2012)

Marker for

Reflects expression of

Fatp1

(Dourlen et al., 2012)

Reporter construct used in assay

Data on Genetic Line

Line ID

l(2)k10307

(Gene Disruption Project members, 2001-)

Origin as a multiple insertion line

Progenitor(s) within the Genome

Related Aberration or Balancer

Aberration

Balancer

Stocks (2)

Bloomington

10988

y¹ w^67c23; P{lacW}Fatp1^k10307/CyO

Kyoto

111287

y^d2 w¹¹¹⁸ P{ey-FLP.N}2 P{5xglBS-lacZ.38-1}TPN1; P{lacW}Fatp1^k10307 P{neoFRT}40A/CyO y⁺

External Crossreferences and Linkouts ( 0 )

Comments

Location 2L:10516565-10516566 confirmed by FlyBase alignment of dbGSS accession AQ034026 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b.

(FlyBase, 2005)

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

P{lacW}Fatp1^k10307

P{lacW}Fatp^k10307

(FlyBase, 2005, FlyBase, 1992-)

P{lacW}l(2)k10307^k10307

(BDGP Project Members, 1994-1999, FlyBase, 1992-)

Name Synonyms

Secondary FlyBase IDs

References (14)

Report Sections

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