FlyBase Insertion Report: Dmel\P{lacW}hth[dtl-S142204]

General Information

Symbol

Dmel\P{lacW}hth^dtl-S142204

Species

D. melanogaster

Name

FlyBase ID

FBti0009704

Feature type

transposable_element_insertion_site

Also Known As

hth-lacZ

Description

Inserted Element

P{lacW}

LINE ID

Affected gene(s)

Ecol\lacZ, hth

Viability / fertility

Associated allele(s)

Ecol\lacZ^{hth-dtl-S142204}, hth^dtl-S142204

Stock availability

1 publicly available

Genomic Location

Chromosomal Location

3R ( 86C2 )

Sequence Location

3R:10,614,316..10,614,318 [-]

Target / Docking site

Genomic Maps

JBrowse

Member of Large Scale Dataset(s)

Dataset

TI_set_P{lacW}.S

Description

A set of third chromosome mutant stocks derived by insertional mutagenesis using the P-element construct P{lacW}; most lines have a lethal or semi-lethal phenotype. The P{lacW} construct carries a w^+mC mini-white visible marker, Ecol\lacZ enhancer trap sequences, and bacterial sequences that allow plasmid rescue (FBrf0049800).

(Deak et al., 1997)

Detailed Mapping Data

Chromosome (arm)

Sequence Location

3R:10,614,316..10,614,318 [-]

(Kurant et al., 1998)

Orientation

Cytological location (computed by FlyBase)

86C2 (inferred by FlyBase from sequence location)

Cytological location (reported)

86C1--86C6 (in situ hybridization reported)

(Deak et al., 1997)

Insertion into Natural transposon

Comments concerning location

Sequence Data

Flanking sequence

Inserted Element

Construct

P{lacW}

Size (Kb)

10.691

Component Allele(s)

w^+mC

Molecular map

Affected Gene(s)

Insertion may affect gene

Ecol\lacZ

(Singh et al., 2011, Grieder et al., 2009, Grieder et al., 2007, Luque and Milan, 2007, Singh et al., 2002, Jaw et al., 2000, Nagao et al., 2000, Yao et al., 1999, Kurant et al., 1998, Pai et al., 1998, Salzberg et al., 1997)

hth

(Raj et al., 2020, Singh et al., 2011, Link et al., 2007, Jaw et al., 1997, Salzberg et al., 1997)

Variant Molecular Consequences

Phenotypic Data

Associated Allele

Phenotypic Data

Ecol\lacZ^{hth-dtl-S142204}

Phenotypic Class Phenotype Manifest in Detailed Description

hth^dtl-S142204

Phenotypic Class Phenotype Manifest in Detailed Description

Expression Data

Reporter Expression

distribution deduced from reporter

Stage

Tissue/Position (including subcellular localization)

Reference

third instar larval stage

ocellus | presumptive

(Pai et al., 1998)

antennal disc

(Pai et al., 1998)

eye disc

(Pai et al., 1998)

photoreceptor neuron

(Pai et al., 1998)

scutum | presumptive

(Pai et al., 1998)

peripodial epithelium

(Pai et al., 1998)

leg disc

(Pai et al., 1998)

ptilinum | presumptive

(Pai et al., 1998)

wing disc

(Pai et al., 1998)

adult head capsule | presumptive

(Pai et al., 1998)

mesothoracic pleurum | presumptive

(Pai et al., 1998)

wing hinge | presumptive

(Pai et al., 1998)

early third instar larval stage

antennal disc | medial | proximal

(Luque and Milan, 2007)

adult head capsule

(Luque and Milan, 2007)

eye-antennal disc | anterior

(Luque and Milan, 2007)

late third instar larval stage

adult head capsule

(Luque and Milan, 2007)

Additional Information

Statement

Reference

The distribution of hth protein and transcripts in wild type imaginal discs is the same as that of lacZ in the enhancer trap line. Enhancer trap expression is observed in the wing disc in the notum, winge hinge, and ventral pleura. In the leg discs, expression is in the periphery region corresponding to the proximal segments of the legs. In the antennal disc, the expression is in all but the arista region. In the eye disc, hth expression is strong in the anterior region surrounding the eye field, including the regions corresponding to the ptilinum, ocellus, and head capsules, and weak in the posterior and lateral margins of the disc. hth is also expressed in all cells of the peripodial membrane in the eye disc. Weak expression is observed in the posterior region composed of mature photoreceptors. This expression pattern generally coincides with that of exd.

(Pai et al., 1998)

hth enhancer trap expression is an excellent reporter of hth gene expression.

(Kurant et al., 1998)

Marker for

Reflects expression of

hth

(Salzberg et al., 1997)

Reporter construct used in assay

Data on Genetic Line

Line ID

Origin as a multiple insertion line

Progenitor(s) within the Genome

Related Aberration or Balancer

Aberration

Balancer

Stocks (1)

Bloomington

78355

w^*; P{lacW}hth^dtl-S142204/TM6B, Tb¹

External Crossreferences and Linkouts ( 0 )

Comments

P{lacW} insertion reported to be 63bp upstream (FBrf100616) or 65 bp upstream (FBrf0101934) of the longest cDNA .

(Kurant et al., 1998)

Synonyms and Secondary IDs (10)

Reported As

Symbol Synonym

P{lacW}dtl^1422-4

(Jaw et al., 1997, FlyBase, 1992-)

P{lacW}hth^dtl-1422-4

(Jaw et al., 1997, FlyBase, 1992-)

P{lacW}hth^dtl-S142204

(Kurant et al., 1998, FlyBase, 1992-)

P{lacW}l(3)S142204^S142204

(FlyBase, 1992-)

hth-lacZ

(Grieder et al., 2009, Grieder et al., 2007, Luque and Milan, 2007, Singh et al., 2002, Jaw et al., 2000, Nagao et al., 2000, Yao et al., 1999)

hth-lacZ^1422-4

(Grieder et al., 2009)

hth^1422-4

(Singh et al., 2011)

hth^1422-4lacZ

(Yao et al., 1999)

hth^dtl-S142204

(Stultz et al., 2012)

l(3)S142204-FRT82

(Link et al., 2007)

Name Synonyms

Secondary FlyBase IDs

FBti0004274
FBti0009545

References (21)

Report Sections

Open Close