FlyBase Insertion Report: Dmel\P{GawB}H24

General Information

Symbol

Dmel\P{GawB}H24

Species

D. melanogaster

Name

FlyBase ID

FBti0016292

Feature type

transposable_element_insertion_site

Also Known As

H24-GAL4, H24

Description

Inserted Element

P{GawB}

LINE ID

Affected gene(s)

Scer\GAL4

Viability / fertility

viable

Associated allele(s)

Scer\GAL4^H24

Stock availability

1 publicly available

Genomic Location

Chromosomal Location

Sequence Location

Target / Docking site

Member of Large Scale Dataset(s)

Dataset

Description

Detailed Mapping Data

Chromosome (arm)

Sequence Location

Orientation

Cytological location (computed by FlyBase)

Cytological location (reported)

Insertion into Natural transposon

Comments concerning location

Sequence Data

Flanking sequence

Inserted Element

Construct

P{GawB}

(Cook, 2013.7.28)

Size (Kb)

11.279

Component Allele(s)

Scer\GAL4^wB

w^+mW.hs

Molecular map

Affected Gene(s)

Insertion may affect gene

Scer\GAL4

(Christiansen et al., 2011, Thum et al., 2011, Chou et al., 2010, Crocker et al., 2010, Pauls et al., 2010, Aso et al., 2009, Pauli et al., 2008, Pauli et al., 2008, Baker et al., 2007, Keleman et al., 2007, Lin et al., 2007, Bhandari et al., 2006, Joiner et al., 2006, Manoli et al., 2005, Suster et al., 2003, Zars et al., 2000, Zars et al., 2000)

Variant Molecular Consequences

Phenotypic Data

Associated Allele

Phenotypic Data

Scer\GAL4^H24

Phenotypic Class Phenotype Manifest in Detailed Description

Expression Data

Reporter Expression

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

larval stage

embryonic/larval mushroom body | restricted

(Suster et al., 2003)

third instar larval stage

inner layer of the larval mushroom body

(Pauls et al., 2010, Pauls et al., 2010)

outer layer of the larval mushroom body

(Pauls et al., 2010, Pauls et al., 2010)

surface layer of the larval mushroom body

(Pauls et al., 2010, Pauls et al., 2010)

adult stage

ellipsoid body

(Zars et al., 2000)

adult antennal lobe projection neuron | subset

(Chou et al., 2010)

Kenyon cell | subset

(Chou et al., 2010)

GABAergic local interneuron of the adult antennal lobe | subset

Comment: 29 of 30 labeled neurons

(Chou et al., 2010)

cholinergic local interneuron of the adult antennal lobe | subset

Comment: 1 of 30 labeled neurons

(Chou et al., 2010)

adult antennal lobe

(Aso et al., 2009, Baker et al., 2007, Zars et al., 2000)

ellipsoid body | restricted

(Baker et al., 2007)

adult antennal lobe local neuron

(Chou et al., 2010)

gamma Kenyon cell

(Christiansen et al., 2011, Pauls et al., 2010)

adult mushroom body | restricted

(Zars et al., 2000)

adult mushroom body gamma-lobe

(Pauls et al., 2010, Aso et al., 2009, Baker et al., 2007, Keleman et al., 2007, Zars et al., 2000)

fan-shaped body

(Aso et al., 2009)

ellipsoid body | intense

(Aso et al., 2009)

medulla | intense

(Aso et al., 2009)

adult lateral horn | faint

(Aso et al., 2009)

adult antennal nerve | intense

(Aso et al., 2009)

adult deutocerebrum

(Aso et al., 2009)

alpha/beta Kenyon cell

(Pauls et al., 2010)

adult pars intercerebralis | faint

(Aso et al., 2009)

adult mushroom body alpha-lobe

(Pauls et al., 2010)

adult mushroom body beta-lobe

(Pauls et al., 2010)

Additional Information

Statement

Reference

Drives expression in gamma lobe Kenyon cells in the mushroom body.

(FlyBase Curators, 2017)

ScerGAL4^H24 labels the mushroom body gamma neurons. Co-expression with presynaptic and postsynaptic markers labels distinct domains of Kenyon cell terminals in the calyx of the mushroom body.

(Christiansen et al., 2011)

Expression of ScerGAL4^H24 is seen in cell bodies found in the local interneuron cluster located lateral and dorsolateral to the antennal lobe. Most labelled cells are ipsilaterally projecting interneurons.

(Chou et al., 2010)

ScerGAL4^H24 drives expression in mostly GABAergic antennal lobe local interneurons, and in a subset of olfactory projection neurons and Kenyon cells.

(Chou et al., 2010)

In the mushroom body, ScerGAL4^H24 drives expression in the whole of the gamma lobe, although with different intensities: the main body of the lobe stains stronger than the dorsomedial tip.

(Aso et al., 2009)

Expression of ScerGAL4^H24 is seen in approximately 40 cells of the ellipsoid body neurons that arborize in the periphery of the ring neurons.

(Baker et al., 2007)

Marker for

adult antennal lobe

(Zars et al., 2000)

adult mushroom body gamma-lobe

(Zars et al., 2000)

antennal lobe

(Zars et al., 2000)

ellipsoid body

(Zars et al., 2000)

gamma Kenyon cell

(Christiansen et al., 2011)

mushroom body

(Zars et al., 2000)

Reflects expression of

Reporter construct used in assay

shi^1.UAS

(Baker et al., 2007)

Ecol\lacZ^UAS.cBa

(Baker et al., 2007)

Ctet\tetX^TNT-LC.UAS

(Suster et al., 2003)

orb2^A.UAS

(Keleman et al., 2007)

Ecol\lacZ^UAS.cUa

(Chou et al., 2010)

Mmus\Cd8a^UAS.cLa.EGFP

(Chou et al., 2010, Pauls et al., 2010, Aso et al., 2009)

Syt1^UAS.Tag:HA

(Chou et al., 2010)

Disc\RFP^{mCh.UAS.Tag:TS(brpS)}

(Christiansen et al., 2011)

Data on Genetic Line

Line ID

Origin as a multiple insertion line

Progenitor(s) within the Genome

Related Aberration or Balancer

Aberration

Balancer

Stocks (1)

Bloomington

51632

P{GawB}H24

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (9)

Reported As

Symbol Synonym

GAL4-H24

(Lin et al., 2007)

H24

(Machado Almeida et al., 2021, Tsai et al., 2018, Bai and Sehgal, 2015, Thum et al., 2011, Pauls et al., 2010, Pauls et al., 2010, Hong et al., 2008, Pavlopoulos et al., 2008, Baker et al., 2007, Bhandari et al., 2006, Joiner et al., 2006, Suster et al., 2003)

H24-GAL4

(Brown et al., 2019, Ries et al., 2017, Crocker et al., 2010, Keleman et al., 2007, Manoli et al., 2005)

H24-Gal4

(Barthel et al., 2024, Delescluse et al., 2024, Zhou et al., 2023, Hatch et al., 2021, Leinwand and Scott, 2021, Lyutova et al., 2019, Sun et al., 2018, Colomb and Brembs, 2016, Kirkhart and Scott, 2015, Pauli et al., 2008, Pauli et al., 2008)

Line 3

(Chou et al., 2010)

P[GAL4]H24

(Martin et al., 1998)

P{GAL4}H24

(Cook, 2013.7.28, Zars et al., 2000, FlyBase, 1992-)

P{GawB}H24

h24-GAL4

(Christiansen et al., 2011)

Name Synonyms

Secondary FlyBase IDs

References (37)

Report Sections

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