A set of transgenic insertion stocks derived by TE mobilization using the P-element construct P{GawB}. The P{GawB} construct carries a w+mW.hs mini-white visible marker and Scer\GAL4 driver/enhancer trap sequences. The GAL4-UAS system is a binary system using Scer\GAL4, a yeast transcription activator protein gene, and Scer\UAS, the DNA binding site for the GAL4 protein. The P{GawB} construct acts as an enhancer trap: expression of the GAL4 is sensitive to enhancers in the genomic region of an insertion. When paired with a construct or insertion carrying a reporter gene downstream of Scer\UAS, the reporter gene is driven by and reflects the expression of the GAL4.
Scer\GAL4NP2211 closely mimics bnl expression at larval and pupal stages, but but fails to drive expression in tracheal placodes at embryonic stages.
ScerGAL4NP2211 is expressed within the tracheal system, specifically by larval tracheal cells along which progenitors migrate. The expression pattern is dynamic and precise, almost perfectly matching the positions and timing of progenitor migration. In L3 animals, when progenitors are observed along the transverse connective (TC) branches, ScerGAL4NP2211 is expressed in TC larval cells in tracheal metameres Tr4 and Tr5, but not in other metameres. Shortly after puparium formation, when pupal abdominal tracheae (PAT) progenitors turn to migrate toward the posterior, larval cells in the segment just posterior to PAT progenitors express ScerGAL4NP2211. As progenitors continue along the dorsal trunk, larval cells in the dorsal trunk activate ScerGAL4NP2211 expression one segment at a time from anterior to posterior, matching progenitor movement.
ScerGAL4NP2211 drives expression in a subpopulation of larval glial cells.
Location 3R:15662628-15662629 refined by FlyBase alignment of dbGSS accessions AG216719 and AG216374 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. Insertion orientation revised.