FlyBase Insertion Report: Dmel\P{GawB}NP4486

General Information

Symbol

Dmel\P{GawB}NP4486

Species

D. melanogaster

Name

FlyBase ID

FBti0035982

Feature type

transposable_element_insertion_site

Description

Inserted Element

P{GawB}

LINE ID

Affected gene(s)

brv1, Scer\GAL4

Viability / fertility

Associated allele(s)

brv1^NP4486, Scer\GAL4^NP4486

Stock availability

1 publicly available

Genomic Location

Chromosomal Location

3L ( 76B3 )

Sequence Location

3L:19,481,491..19,482,128

Target / Docking site

Genomic Maps

JBrowse

Member of Large Scale Dataset(s)

Dataset

TI_set_P{GawB}.NP

Description

A set of transgenic insertion stocks derived by TE mobilization using the P-element construct P{GawB}. The P{GawB} construct carries a w^+mW.hs mini-white visible marker and Scer\GAL4 driver/enhancer trap sequences. The GAL4-UAS system is a binary system using Scer\GAL4, a yeast transcription activator protein gene, and Scer\UAS, the DNA binding site for the GAL4 protein. The P{GawB} construct acts as an enhancer trap: expression of the GAL4 is sensitive to enhancers in the genomic region of an insertion. When paired with a construct or insertion carrying a reporter gene downstream of Scer\UAS, the reporter gene is driven by and reflects the expression of the GAL4.

(Hayashi et al., 2002)

Detailed Mapping Data

Chromosome (arm)

Sequence Location

3L:19,481,491..19,482,128

(FlyBase, 2005)

Orientation

Cytological location (computed by FlyBase)

76B3 (inferred by FlyBase from sequence location)

Cytological location (reported)

Insertion into Natural transposon

Comments concerning location

Sequence Data

Flanking sequence

AG216970

(FlyBase, 1992-)

AG215850

(FlyBase, 1992-)

Inserted Element

Construct

P{GawB}

Size (Kb)

11.279

Component Allele(s)

Scer\GAL4^wB

w^+mW.hs

Molecular map

Affected Gene(s)

Insertion may affect gene

brv1

(Gallio et al., 2011)

Scer\GAL4

(Gallio et al., 2011)

Variant Molecular Consequences

Phenotypic Data

Associated Allele

Phenotypic Data

brv1^NP4486

Phenotypic Class Phenotype Manifest in Detailed Description

Scer\GAL4^NP4486

Phenotypic Class Phenotype Manifest in Detailed Description

Expression Data

Reporter Expression

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

aristal sensory neuron VP3

(Ni et al., 2013, Gallio et al., 2011)

mechanosensory neuron | subset of second segment of antenna

(Gallio et al., 2011)

neuron of sacculus | subset

(Gallio et al., 2011)

antennal lobe glomerulus VP3

(Gallio et al., 2011)

Additional Information

Statement

Reference

Expression detected in three cool-responsive sensory neurons in the arista.

(Ni et al., 2013)

ScerGAL4^brv1-NP4486 labels a subset of mechanosensory neurons of the second antennal segment, three cilitated neurons at the base of the arista which are activated by cold temperatures and which project to the VP3 glomeruli of the antennal lobe. ScerGAL4^brv1-NP4486 also labels around 15 to 20 neurons in the sacculus region.

(Gallio et al., 2011)

Marker for

antennal lobe glomerulus VP3

(Gallio et al., 2011)

Reflects expression of

brv1

(Ni et al., 2013, Gallio et al., 2011)

Reporter construct used in assay

Avic\GFP^{UAS.Tag:NLS(SV40-largeT)}

(Gallio et al., 2011)

Mmus\Cd8a^UAS.cLa.EGFP

(Gallio et al., 2011)