FB2026_02 , released June 18, 2026
Insertion: Dmel\P{GawB}NP5247
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General Information
Symbol
Dmel\P{GawB}NP5247
Species
D. melanogaster
Name
FlyBase ID
FBti0036469
Feature type
transposable_element_insertion_site
Also Known As
miR-8-GAL4, mir-8 gal4, NP5247
Description
Inserted Element
P{GawB}
LINE ID
Affected gene(s)
Scer\GAL4
Viability / fertility
Associated allele(s)
Scer\GAL4NP5247
Stock availability
1 publicly available
Genomic Location
Chromosomal Location
2R ( 53D11 )
Sequence Location
2R:16,830,383..16,830,684
Target / Docking site
Genomic Maps
Member of Large Scale Dataset(s)
Dataset
TI_set_P{GawB}.NP
Description

A set of transgenic insertion stocks derived by TE mobilization using the P-element construct P{GawB}. The P{GawB} construct carries a w+mW.hs mini-white visible marker and Scer\GAL4 driver/enhancer trap sequences. The GAL4-UAS system is a binary system using Scer\GAL4, a yeast transcription activator protein gene, and Scer\UAS, the DNA binding site for the GAL4 protein. The P{GawB} construct acts as an enhancer trap: expression of the GAL4 is sensitive to enhancers in the genomic region of an insertion. When paired with a construct or insertion carrying a reporter gene downstream of Scer\UAS, the reporter gene is driven by and reflects the expression of the GAL4.

(Hayashi et al., 2002)
Detailed Mapping Data
Chromosome (arm)
Sequence Location
2R:16,830,383..16,830,684
(FlyBase, 2005)
Orientation
Cytological location (computed by FlyBase)
53D11 (inferred by FlyBase from sequence location)
Cytological location (reported)
Insertion into Natural transposon
Comments concerning location
Sequence Data
Flanking sequence
AG218094
(FlyBase, 1992-)
Inserted Element
Construct
P{GawB}
Size (Kb)

11.279

Component Allele(s)
Scer\GAL4wB
w+mW.hs
Molecular map
Affected Gene(s)
Insertion may affect gene
Scer\GAL4
(Kennell et al., 2012, Vallejo et al., 2011, Hyun et al., 2009, Loya et al., 2009, Karres et al., 2007)
Variant Molecular Consequences
Phenotypic Data
Expression Data
Reporter Expression
distribution deduced from reporter (Gal4 UAS)
Stage
Tissue/Position (including subcellular localization)
Reference
larval stage
embryonic/larval CNS perineurial glial cell
(Morante et al., 2013)
cell body glial cell
(Morante et al., 2013)
optic-lobe-associated cortex glial cell
(Morante et al., 2013)
third instar larval stage
fat body
(Hyun et al., 2009)
cuticle
(Hyun et al., 2009)
brain | restricted
(Hyun et al., 2009)
adult stage
intestinal stem cell
(Zhai et al., 2015)
enteroblast
(Zhai et al., 2015)
Additional Information
Statement
Reference

ScerGAL4NP5247 drives expression in progenitors (intestinal stem cells and enteroblasts) in the adult midgut.

(Zhai et al., 2015)

ScerGAL4mir-8-NP5247 drives expression in glial cells of the perineurial sheath surrounding the central brain anlage and the developing optic lobes in all larval stages. An average of 137 ScerGAL4mir-8-NP5247 positive glial cells is detected within the optic lobe, with around 25 cells on the surface of the developing optic lobe showing an elaborate arborization pattern. Expression is also driven in a layer of glial cells positioned under the subperineurial glia.

(Morante et al., 2013)
 
Marker for
Reflects expression of
mir-8
(Zhai et al., 2015, Morante et al., 2013)
Reporter construct used in assay
His2BUAS.mRFP1
(Morante et al., 2013)
Mmus\Cd8aUAS.cLa.EGFP
(Morante et al., 2013)
Avic\GFPUAS.cUa
(Hyun et al., 2009)
Data on Genetic Line
Line ID
Origin as a multiple insertion line
Progenitor(s) within the Genome
Related Aberration or Balancer
Aberration
Balancer
Stocks (1)
External Crossreferences and Linkouts ( 1 )
Crossreferences
GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
Synonyms and Secondary IDs (11)
References (14)