A set of transgenic insertion stocks derived by TE mobilization using the P-element construct P{GawB}. The P{GawB} construct carries a w+mW.hs mini-white visible marker and Scer\GAL4 driver/enhancer trap sequences. The GAL4-UAS system is a binary system using Scer\GAL4, a yeast transcription activator protein gene, and Scer\UAS, the DNA binding site for the GAL4 protein. The P{GawB} construct acts as an enhancer trap: expression of the GAL4 is sensitive to enhancers in the genomic region of an insertion. When paired with a construct or insertion carrying a reporter gene downstream of Scer\UAS, the reporter gene is driven by and reflects the expression of the GAL4.
Dendritic branches of class I dendritic arborizing neurons are labeled much more intensely than those of class IV dendritic arborizing neurons by Ten-m-GAL4, and the cell bodies of both classes are almost masked by strong signals from overlying epidermis.
At synapses, ScerGAL4NP6658 is expressed both pre- and post-synaptically. In the ventral nerve cord, ScerGAL4NP6658 is expressed in neurons, including a subset which is also positive for eve, including dorsal motor neurons and some interneurons.
High-level expression of is ScerGAL4NP6658-driven GFP is observed in dorsal oblique muscle 3 and the A1-7 segment border muscle, and basally in all muscles.
Location 3L:22341638-22342236 determined by FlyBase alignment of dbGSS accession AG215084 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. Insertion orientation revised.