FlyBase Insertion Report: Dmel\P{PZ}14-3-3ζ[07103]

General Information

Symbol

Dmel\P{PZ}14-3-3ζ⁰⁷¹⁰³

Species

D. melanogaster

Name

FlyBase ID

FBti0419589

Feature type

transposable_element_insertion_site

Description

Inserted Element

P{PZ}

LINE ID

l(2)07103

Affected gene(s)

14-3-3ζ, Ecol\lacZ

Viability / fertility

viable

Associated allele(s)

14-3-3ζ⁰⁷¹⁰³, Ecol\lacZ^{14-3-3ζ-07103}

Stock availability

1 publicly available

Genomic Location

Chromosomal Location

2R ( 46E6 )

Sequence Location

2R:10,101,498..10,101,498 [+]

Target / Docking site

Genomic Maps

JBrowse

Member of Large Scale Dataset(s)

Dataset

TI_set_P{PZ}.BDGP

Description

Insertion lines from this collection were assessed for inclusion in the Gene Disruption Project collection.

(Bellen et al., 2004)

A set of mutant stocks derived by insertional mutagenesis using the P-element construct P{PZ}; most lines have a lethal or sterile phenotype. The P{PZ} construct carries a ry[+] visible marker, Ecol\lacZ enhancer trap sequences, and bacterial sequences that allow plasmid rescue.

(Spradling et al., 1999)

Detailed Mapping Data

Chromosome (arm)

Sequence Location

2R:10,101,498..10,101,498 [+]

(BDGP Project Members, 1994-1999)

2R:10,101,498..10,101,498 [+]

(BDGP Project Members, 1994-1999)

Orientation

Cytological location (computed by FlyBase)

46E6 (inferred by FlyBase from sequence location)

Cytological location (reported)

46E4-46E8 (in situ hybridization reported)

(BDGP Project Members, 1994-1999)

Insertion into Natural transposon

Comments concerning location

Sequence Data

Flanking sequence

AQ025662

(FlyBase, 1992-)

Y12573

(FlyBase, 1992-)

Inserted Element

Construct

P{PZ}

(FlyBase, 1992-)

Size (Kb)

14.545

Component Allele(s)

l(3)87Df^7.2

ry^+t7.2

Molecular map

Affected Gene(s)

Insertion may affect gene

14-3-3ζ

(Philip et al., 2001, Kockel et al., 1997, FlyBase, 1992-)

Ecol\lacZ

(Jackle and Janning, 1998.8.10, Russell et al., 1998, Vosshall, 1998.8.10, BDGP Project Members, 1994-1999)

Variant Molecular Consequences

Phenotypic Data

Associated Allele

Phenotypic Data

14-3-3ζ⁰⁷¹⁰³

Phenotypic Class Phenotype Manifest in Detailed Description

Ecol\lacZ^{14-3-3ζ-07103}

Phenotypic Class Phenotype Manifest in Detailed Description

Expression Data

Reporter Expression

distribution deduced from reporter

Stage

Tissue/Position (including subcellular localization)

Reference

wandering third instar larval stage

Malpighian tubule

(Meister and Braun, 1995.10)

embryonic/larval hindgut

(Meister and Braun, 1995.10)

larval brain

(Meister and Braun, 1995.10)

imaginal disc

(Meister and Braun, 1995.10)

ring gland

(Meister and Braun, 1995.10)

embryonic/larval foregut

(Meister and Braun, 1995.10)

embryonic/larval midgut

(Meister and Braun, 1995.10)

embryonic/larval salivary gland

(Meister and Braun, 1995.10)

adult stage

adult central complex

(Vosshall, 1998.8.10)

adult brain | restricted

(Vosshall, 1998.8.10)

adult antennal lobe

(Vosshall, 1998.8.10)

adult mushroom body

(Vosshall, 1998.8.10)

Additional Information

Statement

Reference

In wandering third instar larva, moderate levels of expression in brain, ring gland and midgut; low levels in imaginal discs (which discs not specified), foregut, hindgut and Malpighian tubules; very weak expression in salivary gland.

(Meister and Braun, 1995.10)

Marker for

Reflects expression of

14-3-3ζ

(Meister and Braun, 1995.10)

Reporter construct used in assay

Data on Genetic Line

Line ID

l(2)07103

(Gene Disruption Project members, 2001-)

Origin as a multiple insertion line

Progenitor(s) within the Genome

Related Aberration or Balancer

Aberration

Balancer

Stocks (1)

Bloomington

12335

P{PZ}14-3-3ζ⁰⁷¹⁰³ cn¹/CyO; ry⁵⁰⁶

External Crossreferences and Linkouts ( 0 )

Comments

Location 2R:5613311-5613312 confirmed by FlyBase alignment of dbGSS accession AQ025662 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b.

(FlyBase, 2005)

Synonyms and Secondary IDs (7)

Reported As

Symbol Synonym

P{?}14-3-3ζ^P2335

(Philip et al., 2001, FlyBase, 1992-)

P{PZ}14-3-3⁰⁷¹⁰³

(Kockel et al., 1997, FlyBase, 1992-)

P{PZ}14-3-3ζ⁰⁷¹⁰³

(FlyBase, 2005, Kockel et al., 1997, FlyBase, 1992-)

P{PZ}l(2)07103⁰⁷¹⁰³

(FlyBase, 1992-)

P{ry^+t7.2=PZ}l(2)07103⁰⁷¹⁰³

(FlyBase, 1992-)

P{unk}14-3-3ζ^P2335

P{}14-3-3ζ^P2335

(FlyBase, 1992-)

Name Synonyms

Secondary FlyBase IDs

FBti0009122
FBti0000410
FBti0005324
FBti0023266

References (18)

Report Sections

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