FlyBase Allele Report: Dmel\14-3-3ζ[07103]

General Information

Symbol

Dmel\14-3-3ζ⁰⁷¹⁰³

Species

D. melanogaster

Name

FlyBase ID

FBal0408868

Feature type

allele

Associated gene

Dmel\14-3-3ζ

Associated Insertion(s)

P{PZ}14-3-3ζ⁰⁷¹⁰³

Carried in Construct

Also Known As

leo^P2335, leo⁰⁷¹⁰³, l(2)k07103

Key Links

Genomic Maps

JBrowse

Allele class

hypomorphic allele - genetic evidence

Mutagen

P-element activity

Nature of the Allele

Allele class

hypomorphic allele - genetic evidence

(Kockel et al., 1997)

Mutagen

P-element activity

(Kockel et al., 1997, Kockel et al., 1997)

Progenitor genotype

Associated Insertion(s)

P{PZ}14-3-3ζ⁰⁷¹⁰³

Cytology

Description

P{PZ} insertion in the 5' noncoding region.

(Kockel et al., 1997)

P{PZ} insertion into the first intron, 1633bp downstream of the splice donor site of exon I'.

(Kockel et al., 1997)

Allele components

Component

Use(s)

Inserted element

P{PZ}

enhancer trap

Encoded product / tool

lacZ

reporter enzyme

Tagged with

Tag:NLS(P)

nuclear protein localization tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

2R:10,101,498..10,101,498 [+]2R:10,101,498..10,101,498 [+]

(FlyBase, 1992-)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal learning (with 14-3-3ζ^P1188), with 14-3-3ζ^LI.15.hs

(Philip et al., 2001)

abnormal learning (with 14-3-3ζ^P1188), with 14-3-3ζ^LI.15.hs, 14-3-3ζ^LII.2.hs

(Philip et al., 2001)

lethal

(Philip et al., 2001)

lethal (with 14-3-3ζ^P1188)

(Messaritou et al., 2010, Messaritou et al., 2009)

lethal (with Df(2R)X1)

(Goldstein et al., 2001)

lethal | recessive

(Spradling et al., 1999, Kockel et al., 1997)

viable (with 14-3-3ζ^P1188), with 14-3-3ζ^LI.15.hs

(Philip et al., 2001)

viable (with 14-3-3ζ^P1188), with 14-3-3ζ^LI.15.hs, 14-3-3ζ^LII.2.hs

(Philip et al., 2001)

Phenotype Manifest In

eye photoreceptor cell | somatic clone

(Kockel et al., 1997)

Detailed Description

Statement

Reference

12-18% of 14-3-3ζ^P1188/14-3-3ζ^P2335 heteroallelic animals are escapers. They do not exhibit external defects or grossly aberrant behaviors, despite no 14-3-3ζ expression.

(Messaritou et al., 2009)

Flies rescued from lethality by 14-3-3ζ^LI.15.hs or 14-3-3ζ^LI.15.hs and 14-3-3ζ^LII.2.hs show a 25-30% decrement in olfactory learning, comparable to that of (rescued) 14-3-3ζ^2.3. The restoration of learning by the transgenes decays back to mutant levels 60-70 hr later.

(Philip et al., 2001)

Homozygous clones are recovered at low frequency and are small. Clones in the eye frequently lack some photoreceptor cells in the ommatidia.

(Kockel et al., 1997)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhancer of

Statement

Reference

14-3-3ζ⁰⁷¹⁰³ is a non-enhancer of visible phenotype of Scer\GAL4^GMR.PF, btl^λ.UAS, stumps^UAS.Tag:FLAG

(Zhu et al., 2005)

Suppressor of

Statement

Reference

14-3-3ζ⁰⁷¹⁰³ is a suppressor of visible phenotype of H^UAS.cMa, Scer\GAL4^GMR.PF

(Müller et al., 2005)

NOT Suppressor of

Statement

Reference

14-3-3ζ⁰⁷¹⁰³ is a non-suppressor of visible phenotype of Scer\GAL4^GMR.PF, btl^λ.UAS, stumps^UAS.Tag:FLAG

(Zhu et al., 2005)

Phenotype Manifest In

NOT Enhancer of

Statement

Reference

14-3-3ζ⁰⁷¹⁰³ is a non-enhancer of eye phenotype of Scer\GAL4^GMR.PF, btl^λ.UAS, stumps^UAS.Tag:FLAG

(Zhu et al., 2005)

Suppressor of

Statement

Reference

14-3-3ζ⁰⁷¹⁰³ is a suppressor of eye phenotype of H^UAS.cMa, Scer\GAL4^GMR.PF

(Müller et al., 2005)

14-3-3ζ⁰⁷¹⁰³/14-3-3zeta[+] is a suppressor of phenotype of Src42A^Su(Raf)1-1

(Zhang et al., 1999)

NOT Suppressor of

Statement

Reference

14-3-3ζ⁰⁷¹⁰³ is a non-suppressor of eye phenotype of Scer\GAL4^GMR.PF, btl^λ.UAS, stumps^UAS.Tag:FLAG

(Zhu et al., 2005)

Additional Comments

Genetic Interactions

Statement

Reference

Dominantly suppresses the ability of Src42A^Su(phl)1-1 to suppress the lethality of phl¹/Y flies.

(Zhang et al., 1999)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

14-3-3ζ⁰⁷¹⁰³/14-3-3ζ^P1188 is rescued by 14-3-3ζ^I.UAS/Scer\GAL4^elav.PLu

(Messaritou et al., 2009)

14-3-3ζ⁰⁷¹⁰³/14-3-3ζ^P1188 is rescued by 14-3-3ζ^II.UAS/Scer\GAL4^elav.PLu

(Messaritou et al., 2009)

14-3-3ζ⁰⁷¹⁰³/14-3-3ζ^P1188 is rescued by 14-3-3ζ^{III.UAS.Tag:FLAG}/Scer\GAL4^elav.PLu

(Messaritou et al., 2009)

14-3-3ζ⁰⁷¹⁰³/14-3-3ζ^P1188 is rescued by 14-3-3ζ^LI.15.hs

(Philip et al., 2001)

14-3-3ζ^P1375/14-3-3ζ⁰⁷¹⁰³ is rescued by 14-3-3ζ^LI.15.hs

(Philip et al., 2001)

14-3-3ζ⁰⁷¹⁰³ is rescued by 14-3-3ζ^arm.PK

(Kockel et al., 1997)

Partially rescued by

14-3-3ζ⁰⁷¹⁰³/14-3-3ζ^P1188 is partially rescued by Scer\GAL4^elav.PU/14-3-3ζ^{WW.UAS.Tag:FLAG}

(Messaritou et al., 2010)

14-3-3ζ⁰⁷¹⁰³/14-3-3ζ^P1188 is partially rescued by Scer\GAL4^elav.PU/14-3-3ζ^{MW.UAS.Tag:FLAG}

(Messaritou et al., 2010)

Not rescued by

14-3-3ζ⁰⁷¹⁰³/14-3-3ζ^P1188 is not rescued by Scer\GAL4^elav.PU/14-3-3ζ^{WM.UAS.Tag:FLAG}

(Messaritou et al., 2010)

14-3-3ζ⁰⁷¹⁰³/14-3-3ζ^P1188 is not rescued by Scer\GAL4^elav.PU/14-3-3ζ^{MM.UAS.Tag:FLAG}

(Messaritou et al., 2010)

14-3-3ζ⁰⁷¹⁰³ is not rescued by 14-3-3ζ^LI.15.hs

(Philip et al., 2001)

14-3-3ζ⁰⁷¹⁰³ is not rescued by 14-3-3ζ^LII.2.hs

(Philip et al., 2001)

Comments

Expression of 14-3-3ζ^{III.Scer\UAS.T:Zzzz\FLAG} under the control of Scer\GAL4^elav.PLu partially rescues the lethality found in 14-3-3ζ^P1188/14-3-3ζ^P2335 heterozygotes to approximately 70% of expected.

Expression of 14-3-3ζ^II.Scer\UAS under the control of Scer\GAL4^elav.PLu partially rescues the lethality found in 14-3-3ζ^P1188/14-3-3ζ^P2335 heterozygotes to approximately 15-20% of expected.

Expression of 14-3-3ζ^I.Scer\UAS under the control of Scer\GAL4^elav.PLu partially rescues the lethality found in 14-3-3ζ^P1188/14-3-3ζ^P2335 heterozygotes to approximately 20% of expected.

(Messaritou et al., 2009)

The lethality is rescued by 14-3-3ζ^arm.PK.

(Kockel et al., 1997)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

12335

P{PZ}14-3-3ζ⁰⁷¹⁰³ cn¹/CyO; ry⁵⁰⁶

Notes on Origin

Discoverer

A. Spradling.

Comments

Insertion is 2.8kb downstream of the Jra transcription unit.

(Kockel et al., 1997)

Complements: CCS⁰³²²¹. Complements: TER94⁰³⁷⁷⁵. Complements: Pfk⁰⁶³³⁹. Complements: l(2)k03610^k03610. Complements: l(2)k03610^k03703. Complements: l(2)k04308^k04308. Complements: l(2)46Ed^k16104.

(BDGP Project Members, 1994-1999)

External Crossreferences and Linkouts ( 1 )

Crossreferences

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

Y12573

Synonyms and Secondary IDs (12)

Reported As

Symbol Synonym

14-3-3⁰⁷¹⁰³

14-3-3zeta(07103)

(Hansen et al., 2019)

14-3-3ζ⁰⁷¹⁰³

14-3-3ζ^P07103

(Zhu et al., 2005)

14-3-3ζ^P2335

l(2)-07103

(Kockel et al., 1997)

l(2)07103

(Goldstein et al., 2001)

l(2)07103⁰⁷¹⁰³

l(2)K07103

(Zhang et al., 1999)

l(2)k07103

(Spradling et al., 1999)

leo⁰⁷¹⁰³

(Müller et al., 2005, Chen et al., 2003)

leo^P2335

(Messaritou et al., 2010, Messaritou et al., 2009, Philip et al., 2001)

Name Synonyms

Secondary FlyBase IDs

FBal0008125
FBal0134434

References (16)

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