FlyBase Recombinant Construct Report: P{shakB(lethal)-GAL4.4.1}

General Information

Symbol

P{shakB(lethal)-GAL4.4.1}

FlyBase ID

FBtp0013641

Feature type

transgenic_transposable_element

Component Allele(s)

Scer\GAL4^{shakB.lethal.4.1}

Also Known As

shakB-gal4, shakB(lethal)-Gal4

Size

Expression Data

GAL4 reporter/driver

Associated insertion(s)

2 available

Molecular map

Description and Uses

Construct components

Component

Use(s)

Marker(s)

w^+mW.hs

Component allele Scer\GAL4^{shakB.lethal.4.1}

Transgenic product class

Component

Use(s)

Regulatory region(s)

shakB

Encoded product / tool

GAL4

binary expression system - driver

Description

A 4.1kb HindIII-EcoRI region from the shakB(lethal) promoter regulates expression of a GAL4 driver.

(Jacobs et al., 2000)

Sequence Data

Sequence (FB)

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA Sequence

Extent

Segments and Size

Total Size

Left end

Right end

Segments

Num

Orientation

Symbol

Reference

Phenotypic Data

Component Allele

Phenotypic Data

Scer\GAL4^{shakB.lethal.4.1}

Phenotypic Class Phenotype Manifest In Detailed Description

Expression Data

Reporter Expression

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

third instar larval stage

larval RP2 motor neuron

(Gao et al., 2013, Freeman et al., 2011)

wandering third instar larval stage

type Is motor neuron

(Newman et al., 2017)

prepupal stage P4 -- adult stage

mesothoracic tergotrochanter muscle motor neuron

(Jacobs et al., 2000)

pupal stage

mesothoracic tergotrochanter muscle motor neuron

(Godenschwege et al., 2002)

peripherally synapsing interneuron

(Godenschwege et al., 2002)

adult stage

mesothoracic tergotrochanter muscle motor neuron

(Bloomington Drosophila Stock Center, 2017, Lee and Godenschwege, 2015)

Additional Information

Statement

Reference

ScerGAL4^{shakB.lethal.4.1} drives expression in tergotrochanteral (jump) muscle motoneuron and other neurons.

(Bloomington Drosophila Stock Center, 2017)

Drives expression in tergotrochanteral muscle motor neuron, and in peripherally synapsing interneurons, and in larval RP2 and type Is motor neurons.

(FlyBase Curators, 2017)

ScerGAL4^{shakB.lethal.4.1} drives expression in the tergotrochanteral muscle motor neuron, a postsynaptic target of the giant fiber neuron, but not in the giant fiber neuron.

(Lee and Godenschwege, 2015)

ScerGAL4^{shakB.lethal.4.1} drives expression in the tergotrochanteral muscle motor neuron (TTMn), and peripherally synapsing interneuron (PSI). and dorsal longitudinal motor neuron, but not in the giant fiber neuron.

(Godenschwege et al., 2002)

ScerGAL4^{shakB.lethal.4.1} drives expression in relatively few neurons in the CNS, most prominently in the tergotrochanteral muscle motor neuron (TTMn), but not in the giant fiber neuron. Pupal expression is also observed in several neurons in the optic lobes, central brain, subesophageal ganglion, and the thoracico-abdominal ganglion.

(Jacobs et al., 2000)

Marker for

Reflects expression of

shakB

(Newman et al., 2017, Gao et al., 2013, Freeman et al., 2011, Godenschwege et al., 2002, Jacobs et al., 2000)

Reporter construct used in assay

Progenitors and Descendants

progenitor(s)

descendant(s)

Comments

Stocks (1)

Stocks containing insertion(s) of this recombinant construct

Bloomington

51633

y¹ w^*; P{shakB(lethal)-GAL4.4.1}2GL152

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (15)

Reported As

Symbol Synonym

P[shakB-Gal4]

(Murphey et al., 2003)

P{shakB(lethal)-GAL4.4.1}

(FlyBase, 1996-)

P{shakB-GAL4}

(Lima and Miesenbock, 2005)

Shak-B-GAL4

(Storkebaum et al., 2009)

ShakB

(Freeman et al., 2011)

ShakB Gal4

(Lee and Godenschwege, 2015)

ShakB-GAL4

(Gao et al., 2013)

ShakB-Gal4

(Bulgari et al., 2019, Lee et al., 2018, Newman et al., 2017, Uthaman et al., 2008)

ShakB:GAL4

(Hsu et al., 2020)

Shaking B GAL4

(Banerjee et al., 2004)

ShakingB-GAL4

(Yoon et al., 2017)

shakB(lethal)-Gal4

(Godenschwege et al., 2002, Jacobs et al., 2000)

shakB-GAL4

(Bogaert et al., 2018)

shakB-Gal4

(Genç and Davis, 2019, Ermanoska et al., 2014, Enneking et al., 2013)

shakB-gal4

(Godenschwege et al., 2006)

Secondary FlyBase IDs

References (22)

Report Sections

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