The P{αTub84B(FRT.Myc)GAL4.Bb} construct contains the αTub84B promoter fused to FRT sequences, followed by the entire open reading frame of Myc, followed by the y open reading frame fused to a tubulin trailer, followed by FRT sequences and sequence encoding a GAL4 driver.
The P{αTub84B(FRT.Myc)GAL4.Bb} construct contains the αTub84B promoter fused to FRT sequences, followed by the entire open reading frame of Myc, followed by the y open reading frame fused to a tubulin trailer, followed by FRT sequences and sequence encoding a GAL4 driver.
The αTub84B promoter is separated from sequence encoding a GAL4 driver by an FRT cassette that contains the entire open reading frame of Myc, followed by a y+t* marker and a tubulin trailer.
Designed for use in mosaic analyses; transcription blocked by FRT-flanked fragment carrying Myc followed by y+M. See expression pattern for ScerGAL4αTub84B.PBb, carried in descendant transposon P{αTub84B(-FRT)GAL4.Bb}.