A synthetic oligo (CTAGTCTAGACTAG) has introduced stop codons in all 3 reading frames at +502 bp relative to the common transcription initiation site of Tgs1 and moi. This introduces a stop codon into moi, but does not affect the sequence of Tgs1.
A synthetic oligo (CTAGTCTAGACTAG) has introduced stop codons in all 3 reading frames at +502 bp relative to the common transcription initiation site of Tgs1 and moi. This introduces a stop codon into moi, but does not affect the sequence of Tgs1.